Nagle R B, Ahmann F R, McDaniel K M, Paquin M L, Clark V A, Celniker A
Cancer Res. 1987 Jan 1;47(1):281-6.
Two murine monoclonal anti-cytokeratin antibodies with defined specificity were shown to distinguish between basal cells and luminal cells in human prostate tissue. Forty-one biopsies or transurethral resection specimens were characterized using these two antibodies. In cases of benign prostatic hyperplasia, focal loss of the basal cell layer was noted in areas of glandular proliferation. Ten cases of adenocarcinoma of the prostate, varying in Gleason's histological grade from 2 to 4, were also studied. In each case the carcinoma was shown to represent the luminal cell phenotype with no evidence of involvement of the basal cell phenotype. An analysis of three established metastatic prostatic carcinoma cell lines (DU-145, PC-3, and LNCaP) using two-dimensional electrophoresis showed that the cytokeratin complement of each cell line was slightly different but retained the phenotype of the luminal cell. It was concluded that during both hyperplasia and neoplastic transformation of the prostate, the luminal cell phenotype is primarily involved and that the basal cell phenotype does not appear to contribute to either intraluminal proliferation or invasive cell populations.
两种具有明确特异性的鼠单克隆抗细胞角蛋白抗体被证明可区分人前列腺组织中的基底细胞和管腔细胞。使用这两种抗体对41份活检或经尿道切除标本进行了特征分析。在良性前列腺增生病例中，在腺体增生区域发现基底细胞层局灶性缺失。还研究了10例前列腺腺癌，Gleason组织学分级从2到4不等。在每例病例中，癌均表现为管腔细胞表型，无基底细胞表型受累的证据。使用二维电泳对三种已建立的转移性前列腺癌细胞系（DU-145、PC-3和LNCaP）进行分析表明，每个细胞系的细胞角蛋白组成略有不同，但保留了管腔细胞的表型。得出的结论是，在前列腺增生和肿瘤转化过程中，主要涉及管腔细胞表型，而基底细胞表型似乎对管腔内增殖或侵袭性细胞群体均无贡献。
