Zagury D, Fouchard M, Cheynier R, Bernard J, Cattan A, Salahuddin S Z, Sarin P S
Cancer Res. 1985 Sep;45(9 Suppl):4595s-4597s.
The development of acquired immunodeficiency syndrome or of acquired immunodeficiency syndrome-related complex by transmission of human T-lymphotropic retrovirus III by semen has previously been implicated by epidemiological studies. In vitro investigations were performed on mononuclear cells obtained from the semen of patients with acquired immunodeficiency syndrome to identify human T-lymphotropic retrovirus III or related retrovirus. The presence of human T-lymphotropic retrovirus III was demonstrated (a) in primary cell cultures, by the detection of the Mr 24,000 protein by indirect immunofluorescence assays by Day 6; (b) in activated long-term cell culture by reverse transcriptase activity, by indirect immunofluorescence (Mr 24,000 protein); and (c) in cocultures of T-cells from semen of AIDS patients and H9 cells by reverse transcriptase activity, indirect immunofluorescence, and the presence of virus particles by electron microscopy.
此前，流行病学研究表明，通过精液传播人类嗜T淋巴细胞逆转录病毒III可导致获得性免疫缺陷综合征或与获得性免疫缺陷综合征相关的综合征的发生。对从获得性免疫缺陷综合征患者精液中获取的单核细胞进行了体外研究，以鉴定人类嗜T淋巴细胞逆转录病毒III或相关逆转录病毒。通过以下方式证实了人类嗜T淋巴细胞逆转录病毒III的存在：（a）在原代细胞培养物中，在第6天通过间接免疫荧光测定法检测到分子量为24,000的蛋白质；（b）在活化的长期细胞培养物中，通过逆转录酶活性、间接免疫荧光（分子量为24,000的蛋白质）；（c）在艾滋病患者精液中的T细胞与H9细胞的共培养物中，通过逆转录酶活性、间接免疫荧光以及电子显微镜观察到病毒颗粒。
