Folks T, Kelly J, Benn S, Kinter A, Justement J, Gold J, Redfield R, Sell K W, Fauci A S
J Immunol. 1986 Jun 1;136(11):4049-53.
Normal human peripheral blood lymphocytes were tested for their susceptibility to infection with retroviruses isolated from patients with the acquired immunodeficiency syndrome (AIDS) or AIDS-related complex. Of 10 normal individuals tested, lymphocytes from all subjects became infected and produced virus as detected by assay for Mg+2-dependent reverse transcriptase. Lymphocytes from different individuals were demonstrated to be either high or low producers of reverse transcriptase after infection. The kinetics of virus production were similar in cells from both high- and low-producing individuals. A significant correlation was observed between high and low viral-producing lymphocytes and expression of the Leu-3/T4 (CD4) surface molecule. Mitogen-stimulated peripheral blood lymphocytes exposed to HTLV-III/LAV manifested productive viral infection, as reflected by the appearance of early syncytia, followed by reverse transcriptase. Unstimulated peripheral blood lymphocyte cultures displayed late syncytia but no detectable reverse transcriptase upon exposure to virus. The addition of anti-human interferon-alpha did not appear to have an appreciable effect on viral production in normal peripheral blood lymphocytes exposed to the virus.
对正常人类外周血淋巴细胞进行检测，以确定其对从获得性免疫缺陷综合征（AIDS）或AIDS相关综合征患者中分离出的逆转录病毒的易感性。在接受检测的10名正常个体中，所有受试者的淋巴细胞均被感染并产生病毒，这通过镁离子依赖性逆转录酶检测得以证实。感染后，不同个体的淋巴细胞被证明是逆转录酶的高产者或低产者。高产和低产个体的细胞中病毒产生的动力学相似。高产和低产病毒的淋巴细胞与Leu-3/T4（CD4）表面分子的表达之间存在显著相关性。暴露于HTLV-III/LAV的丝裂原刺激外周血淋巴细胞表现出有生产性的病毒感染，这表现为早期多核巨细胞的出现，随后是逆转录酶的出现。未刺激的外周血淋巴细胞培养物在暴露于病毒后显示出晚期多核巨细胞，但未检测到逆转录酶。添加抗人α干扰素似乎对暴露于该病毒的正常外周血淋巴细胞中的病毒产生没有明显影响。
