鸡肌间神经丛发育过程中氮能神经元的定量变化。
Quantitative changes of nitrergic neurons during postnatal development of chicken myenteric plexus.
机构信息
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Department of Anatomy and Histology, Sindh Agriculture University, Tandojam 70060, Pakistan.
出版信息
J Zhejiang Univ Sci B. 2013 Oct;14(10):886-95. doi: 10.1631/jzus.B1300005.
OBJECTIVE
Information regarding the development of the enteric nervous system (ENS) is important for understanding the functional abnormalities of the gut. Because fertilized chicken eggs provide easy access to embryos, chicken models have been widely used to study embryonic development of myenteric plexus; however, no study has been focused on the postnatal period. The aim of this study was to perform a qualitative and quantitative analysis of the nitrergic neurons in the myenteric plexus of developing chickens in the postnatal period.
METHODS
Whole-mount preparations of the myenteric plexus were made in 7-d, 15-d, and 40-d old (adult) chickens of either sex (n=15). The myenteric plexus was studied after nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry using light microscopy, digital photography, and Image-Pro Plus 6.0 software. The numbers of positively stained neurons and ganglia were counted in the duodenum, jejunum, ileum, caecum, and colon in the different age groups. Data were expressed as mean±standard deviation (SD), and statistical analysis was performed using a one-way analysis of variance (ANOVA) test.
RESULTS
The positively stained neurons showed various morphologies and staining intensities, and formed bead-shaped and U-shaped arrangements in the myenteric plexus. The densities of neurons and ganglia increased with age. However, the number of positive neurons per ganglion increased. The number of NADPH-d-positive neurons was highest in the colon, followed by the ileum, the jejunum, the duodenum, and the caeca in all age groups.
CONCLUSIONS
Developmental changes in the myenteric plexus of chickens continue in the postnatal period, indicating that the maturation process of the gastrointestinal function is gradual. In addition, no significant difference is happening among different intestinal segments during postnatal development, suggesting that the function of different intestinal segments had been determined after birth.
目的
了解肠神经系统（ENS）的发育信息对于理解肠道的功能异常很重要。由于受精鸡卵可方便地获得胚胎，鸡模型已被广泛用于研究肌间神经丛的胚胎发育；然而，尚无研究集中于出生后时期。本研究旨在对出生后不同日龄（7 日龄、15 日龄和 40 日龄，即成年）的雌雄鸡的肌间神经丛中的氮能神经元进行定性和定量分析。
方法
使用烟酰胺腺嘌呤二核苷酸磷酸二氢酶（NADPH-d）组织化学法，对 7 日龄、15 日龄和 40 日龄（成年）的雌雄鸡的整个肌间神经丛进行全膜制备。在不同年龄组的十二指肠、空肠、回肠、盲肠和结肠中，使用光学显微镜、数字摄影和 Image-Pro Plus 6.0 软件对 NADPH-d 阳性神经元和神经节的数量进行研究。数据以均数±标准差（SD）表示，并使用单因素方差分析（ANOVA）检验进行统计学分析。
结果
阳性染色神经元具有不同的形态和染色强度，并在肌间神经丛中形成珠状和 U 形排列。神经元和神经节的密度随年龄的增加而增加。然而，每个神经节的阳性神经元数量增加。在所有年龄组中，NADPH-d 阳性神经元数量最多的部位是结肠，其次是回肠、空肠、十二指肠和盲肠。
结论
鸡肌间神经丛的发育变化在出生后持续进行，表明胃肠道功能的成熟过程是逐渐的。此外，在出生后发育过程中不同肠段之间没有明显差异，这表明不同肠段的功能在出生后已经确定。
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