Gustafsson B, Holme T
Eur J Clin Microbiol. 1985 Jun;4(3):291-4. doi: 10.1007/BF02013655.
Monoclonal antibodies against the group and type specific antigens of Vibrio cholerae O:1 lipopolysaccharide were used for the rapid detection of Vibrio cholerae strains by motility inhibition and immunofluorescence. Motility inhibition of live Vibrio cholerae O:1 was obtained with group specific monoclonal antibodies. Monoclonal antibodies against the type specific antigens B (Ogawa) and C (Inaba) inhibited motility of strains of homologous serotypes only. Indirect immunofluorescence of heatfixed bacteria with monoclonal antibodies and fluorescein-isothiocyanate conjugated rabbit anti-mouse immunoglobulin was also shown to be suitable for the rapid detection of Vibrio cholerae O:1. Both tests were highly specific and no cross-reactions were observed with strains of non-O:1 vibrios, Escherichia coli or Salmonella spp. tested. However, a weak fluorescence of some Ogawa strains was observed when high concentrations of Inaba specific monoclonal antibodies were used.
针对霍乱弧菌O:1脂多糖的群特异性和型特异性抗原的单克隆抗体，被用于通过运动抑制和免疫荧光法快速检测霍乱弧菌菌株。用群特异性单克隆抗体可实现对活的霍乱弧菌O:1的运动抑制。针对型特异性抗原B（小川型）和C（稻叶型）的单克隆抗体仅抑制同源血清型菌株的运动。用单克隆抗体和异硫氰酸荧光素偶联的兔抗小鼠免疫球蛋白对热固定细菌进行间接免疫荧光检测，也被证明适用于快速检测霍乱弧菌O:1。这两种检测方法都具有高度特异性，在所检测的非O:1弧菌、大肠杆菌或沙门氏菌菌株中未观察到交叉反应。然而，当使用高浓度的稻叶型特异性单克隆抗体时，观察到一些小川型菌株有微弱荧光。
