Qadri F, Azim T, Chowdhury A, Hossain J, Sack R B, Albert M J
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Clin Diagn Lab Immunol. 1994 Jan;1(1):51-4. doi: 10.1128/cdli.1.1.51-54.1994.
Mouse monoclonal antibodies (MAbs) were derived against acetone-treated whole cells of the newly recognized Vibrio cholerae O139 serogroup which is causing epidemics of cholera-like disease in India and Bangladesh. Four MAbs specifically recognized the lipopolysaccharide antigens of V. cholerae O139. MAbs ICL9 and ICL13 were of the immunoglobulin M (IgM) isotype, ICL11 was of the IgG3 isotype, and ICL12 was of the Ig2b isotype. A fifth MAb, ICL10, of the IgG2b isotype cross-reacted with V. cholerae O91. All five MAbs recognized V. cholerae O139 in an enzyme-linked immunosorbent assay, slide agglutination test, motility inhibition test, and indirect immunofluorescence test. During a 1-month evaluation of these MAbs in our clinical laboratory, all 86 cases diagnosed as V. cholerae O139 by a rabbit polyclonal antiserum were also detected by these MAbs, establishing their utility as highly sensitive and specific diagnostic reagents. With these MAbs, it should now be possible to screen for the V. cholerae O139 serogroup in epidemic and endemic diarrhea cases and in environmental and food samples.
鼠单克隆抗体（MAbs）是针对经丙酮处理的新发现的霍乱弧菌O139血清群全细胞产生的，该血清群正在印度和孟加拉国引发霍乱样疾病的流行。四种单克隆抗体特异性识别霍乱弧菌O139的脂多糖抗原。单克隆抗体ICL9和ICL13属于免疫球蛋白M（IgM）同种型，ICL11属于IgG3同种型，ICL12属于Ig2b同种型。第五种单克隆抗体ICL10属于IgG2b同种型，与霍乱弧菌O91发生交叉反应。所有五种单克隆抗体在酶联免疫吸附测定、玻片凝集试验、动力抑制试验和间接免疫荧光试验中均能识别霍乱弧菌O139。在我们临床实验室对这些单克隆抗体进行的为期1个月的评估期间，所有86例经兔多克隆抗血清诊断为霍乱弧菌O139的病例也被这些单克隆抗体检测到，证实了它们作为高度敏感和特异的诊断试剂的效用。有了这些单克隆抗体，现在应该能够在流行和地方性腹泻病例以及环境和食品样本中筛查霍乱弧菌O139血清群。
