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基于植物生产两种针对霍乱弧菌脂多糖免疫显性表位的嵌合单克隆IgG抗体。

Plant-based production of two chimeric monoclonal IgG antibodies directed against immunodominant epitopes of Vibrio cholerae lipopolysaccharide.

作者信息

Levinson Kara J, Giffen Samantha R, Pauly Michael H, Kim Do H, Bohorov Ognian, Bohorova Natasha, Whaley Kevin J, Zeitlin Larry, Mantis Nicholas J

机构信息

Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, United States; Department of Biomedical Sciences, University at Albany, Albany, NY 12208, United States.

Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY 12208, United States.

出版信息

J Immunol Methods. 2015 Jul;422:111-7. doi: 10.1016/j.jim.2015.04.001. Epub 2015 Apr 9.

DOI:10.1016/j.jim.2015.04.001
PMID:25865265
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4458452/
Abstract

We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. Both antibodies were expressed using a Nicotiana benthamiana-based rapid antibody-manufacturing platform (RAMP) and evaluated in vitro for activities associated with immunity to V. cholerae, including vibriocidal activity, bacterial agglutination and motility arrest.

摘要

我们制备并鉴定了两种嵌合人IgG1单克隆抗体，它们可结合霍乱弧菌脂多糖（LPS）上不同的免疫显性表位。单克隆抗体2D6 IgG1识别小川型O-多糖抗原，而单克隆抗体ZAC-3 IgG1识别小川型和稻叶型LPS的核心/脂质A部分。两种抗体均使用基于本氏烟草的快速抗体制备平台（RAMP）表达，并在体外评估了与霍乱弧菌免疫相关的活性，包括杀弧菌活性、细菌凝集和运动抑制。

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