Jia Shangang, Zhang Xiaowei, Zhang Guangyu, Yin An, Zhang Sun, Li Fusen, Wang Lei, Zhao Duojun, Yun Quanzheng, Wang Jixiang, Sun Gaoyuan, Baabdullah Mohammed, Yu Xiaoguang, Hu Songnian, Al-Mssallem Ibrahim S, Yu Jun
Joint Center for Genomics Research, King Abdulaziz City for Science and Technology and Chinese Academy of Sciences, Riyadh, Kingdom of Saudi Arabia.
CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
Environ Microbiol. 2013 Nov;15(11):3020-9. doi: 10.1111/1462-2920.12262. Epub 2013 Oct 9.
The intestinal microbes residing in the red palm weevil (RPW, Rhynchophorus ferrugineus) larva consume tender interior fibrous tissues of date palm trunks. The understanding of such microbiota at molecular level provides vital clues for the biological control of this devastating pest. Using pyrosequencing and shotgun strategy, we first study taxonomic profiles of the microbiota sampled at different months (March, July and November), and then confirm the impact of high-temperature stress on the microbial populations based on data from 16S rRNA amplicons using both field and laboratory samples. We further identify Klebsiella pneumoniae in November and Lactococcus lactis in July as the dominant species of the microbiota. We find that the RPW gut microbiota degrades polysaccharides and sucrose with hydrolases and that different active bacterial species in November and July are responsible for the symbiotic relationship between the microbiota and the host. Our results provide vital information for pest control and cellulolytic bacterial species characterization.
红棕象甲（RPW，锈色棕榈象）幼虫体内的肠道微生物会消耗枣椰树干内部的嫩纤维组织。在分子水平上了解这种微生物群为生物防治这种毁灭性害虫提供了重要线索。我们首先使用焦磷酸测序和鸟枪法策略研究了不同月份（3月、7月和11月）采集的微生物群的分类概况，然后基于来自田间和实验室样本的16S rRNA扩增子数据，证实了高温胁迫对微生物种群的影响。我们进一步确定11月的肺炎克雷伯菌和7月的乳酸乳球菌为微生物群的优势种。我们发现，红棕象甲肠道微生物群通过水解酶降解多糖和蔗糖，11月和7月不同的活性细菌物种负责微生物群与宿主之间的共生关系。我们的结果为害虫防治和纤维素分解细菌物种表征提供了重要信息。
