Van Der Poel W H M, Parlevliet J M, Verstraten E R A M, Kooi E A, Hakze-Van Der Honing R, Stockhofe N
Central Veterinary Institute (CVI) of Wageningen University and Research Centre,Lelystad,The Netherlands.
Faculty of Veterinary Medicine,Utrecht University,The Netherlands.
Epidemiol Infect. 2014 Jul;142(7):1495-500. doi: 10.1017/S0950268813002574. Epub 2013 Oct 9.
To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT-PCR). After SBV infection both animals in the study showed viraemia (qRT-PCR) with fever and diarrhoea. SBV RNA could be detected in semen from both animals. The highest SBV RNA concentrations in semen were found in the first week (days 4-7 post-inoculation) but concentrations were relatively low (Ct values 30-39). Viable SBV was only isolated from blood samples and not from semen or genital tissues.
为研究实验感染后牛精液中施马伦贝格病毒（SBV）的排泄情况，对两头公牛进行皮下接种SBV分离株（1毫升含有106半数组织培养感染剂量的Vero细胞培养物）。接种后（第0天），每天从这两头动物采集精液，持续21天，并通过定量逆转录聚合酶链反应（qRT-PCR）检测样本中的SBV。接种后第24天，对两头动物进行尸检，并对生殖器官及引流这些器官的淋巴结进行SBV RNA检测（qRT-PCR）。SBV感染后，研究中的两头动物均出现病毒血症（qRT-PCR检测），伴有发热和腹泻。在两头动物的精液中均可检测到SBV RNA。精液中SBV RNA的最高浓度出现在第一周（接种后第4 - 7天），但浓度相对较低（Ct值为30 - 39）。仅从血液样本中分离出活的SBV，精液或生殖组织中未分离出。
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