Adam Ahmed A M, Amine Ahmed A A, Hassan Dina A, Omer Waleed H, Nour Bakri Y, Jebakumar Arulanantham Zechariah, Ibrahim Muntaser E, Abdulhadi Nasreldin H, Mohamed Hiba S
Institute of Endemic Diseases, University of Khartoum, P,O, Box 102, Khartoum, Sudan.
BMC Infect Dis. 2013 Oct 9;13:469. doi: 10.1186/1471-2334-13-469.
The Erythrocyte Binding Antigen (EBA) 175 has been considered as one of the most important Plasmodium falciparum (P. falciparum) merozoite ligands that mediate invasion of the erythrocytes through their sialated receptor: Glycophorin A (GPA). The effect of the EBA 175 dimorphic alleles (F and C) on the severity of the disease is not yet fully understood. Therefore this study was designed to assess the distribution of the divergent dimorphic alleles of P. falciparum EBA-175 (F and C) in three different geographical areas in Sudan and the possible association of this dimorphism with the severity of the disease.
A sum of 339 field isolates of P. falciparum obtained from patients in three different geographical areas in Sudan were screened for the dimorphic alleles (F, C) of the EBA-175 using nested PCR.
The percentage of F, C, and mixed F/C alleles were; 41%, 51%, and 8% respectively. F and C alleles showed significantly different distributions in the various geographic areas (p = 0.00). There was no significant association between malaria clinical manifestation and P. falciparum EBA-175 F and C alleles frequencies.
This study showed a significant differential distribution of F and C alleles in different geographical malaria endemic areas. No significant association was observed between F and C alleles and different malaria phenotypes.
红细胞结合抗原（EBA）175被认为是恶性疟原虫最重要的裂殖子配体之一，它通过其唾液酸化受体血型糖蛋白A（GPA）介导对红细胞的入侵。EBA 175双态等位基因（F和C）对疾病严重程度的影响尚未完全明确。因此，本研究旨在评估苏丹三个不同地理区域中恶性疟原虫EBA - 175（F和C）不同双态等位基因的分布情况，以及这种双态性与疾病严重程度之间可能存在的关联。
使用巢式PCR对从苏丹三个不同地理区域的患者中获得的总共339株恶性疟原虫野外分离株进行EBA - 175双态等位基因（F、C）的筛查。
F、C和混合F/C等位基因的百分比分别为41%、51%和8%。F和C等位基因在各个地理区域的分布存在显著差异（p = 0.00）。疟疾临床表现与恶性疟原虫EBA - 175 F和C等位基因频率之间没有显著关联。
本研究表明F和C等位基因在不同地理疟疾流行区存在显著的差异分布。未观察到F和C等位基因与不同疟疾表型之间存在显著关联。
