Ackermans F, Klein J P, Cormont F, Bazin H, Ogier J A, Frank R M, Vreven J
Infect Immun. 1985 Aug;49(2):344-50. doi: 10.1128/iai.49.2.344-350.1985.
Monoclonal antibodies to Streptococcus mutans OMZ175 (serotype f) cell wall-associated antigens (wall-extracted antigens [WEA]) were derived from the fusion of Lou C plasmocytoma rat cells (IR 983 F) and spleen cells from Wistar R inbred rats immunized with WEA. Four cell lines producing monoclonal antibodies directed against a component of S. mutans WEA have been established. All four monoclonal antibodies reacted only with two antigens of WEA from S. mutans OMZ175 by Western blotting and immunoprecipitation techniques, enzyme-linked immunosorbent assay (ELISA), and competitive ELISA. Western blot analysis of WEA showed that the four monoclonal antibodies recognized two related cell wall-associated proteins with apparent molecular weights of 125,000 and 76,000. Immunoprecipitation of whole cells with the monoclonal antibodies confirmed the surface localization of the two antigens. The ELISA and competitive ELISA were used to analyze the distribution of the epitopes on seven S. mutans serotypes. All S. mutans serotypes were found to express the recognized epitopes; however, different reactivity patterns could be distinguished among the various strains tested, and the four monoclonal antibodies reacted only weakly with S. mutans serotypes d and g.
针对变形链球菌OMZ175（血清型f）细胞壁相关抗原（细胞壁提取抗原[WEA]）的单克隆抗体，源自Lou C浆细胞瘤大鼠细胞（IR 983 F）与用WEA免疫的Wistar R近交系大鼠的脾细胞的融合。已经建立了四个产生针对变形链球菌WEA组分的单克隆抗体的细胞系。通过蛋白质免疫印迹和免疫沉淀技术、酶联免疫吸附测定（ELISA）和竞争性ELISA，所有四种单克隆抗体仅与变形链球菌OMZ175的WEA的两种抗原发生反应。对WEA的蛋白质免疫印迹分析表明，这四种单克隆抗体识别出两种表观分子量分别为125,000和76,000的相关细胞壁相关蛋白。用单克隆抗体对全细胞进行免疫沉淀证实了这两种抗原的表面定位。ELISA和竞争性ELISA用于分析七种变形链球菌血清型上表位的分布。发现所有变形链球菌血清型均表达所识别的表位；然而，在所测试的各种菌株之间可以区分出不同的反应模式，并且这四种单克隆抗体与变形链球菌血清型d和g的反应较弱。
