用针对变形链球菌细胞壁抗原的单克隆抗体识别碳水化合物和蛋白质表位
Recognition of carbohydrate and protein epitopes by monoclonal antibodies to a cell wall antigen from Streptococcus mutans.
作者信息
Mitchell C G, Smith R, Lehner T
出版信息
Infect Immun. 1987 Mar;55(3):810-5. doi: 10.1128/iai.55.3.810-815.1987.
Abstract
The nature of the determinants recognized by a panel of monoclonal antibodies (MAbs) raised against a cell wall antigen of Streptococcus mutans (SA I/II) was investigated. Mild periodate oxidation of SA I/II showed that MAbs Guy 1, 2, 3, and 5 recognized carbohydrate epitopes on the antigen. Glycosidases were used to identify the nature of the sugars involved in their binding. Treatment with beta-glucosidase inhibited the binding of Guy 1, 2, 3, and 5 by 90%. No competition was found for any of the MAbs between SA I/II and a series of carbohydrates, including the serotype c polysaccharide from S. mutans. The results show that MAbs Guy 1, 2, 3, and 5 recognize carbohydrate epitopes on SA I/II which are distinct from the serotype polysaccharide. The other MAbs recognized protein epitopes on SA I/II.
摘要
研究了一组针对变形链球菌细胞壁抗原（SA I/II）产生的单克隆抗体（MAb）所识别的决定簇的性质。SA I/II的轻度高碘酸盐氧化表明，MAb Guy 1、2、3和5识别抗原上的碳水化合物表位。糖苷酶用于鉴定参与其结合的糖的性质。用β-葡萄糖苷酶处理可使Guy 1、2、3和5的结合抑制90%。在SA I/II与一系列碳水化合物（包括变形链球菌c型多糖）之间，未发现任何MAb存在竞争。结果表明，MAb Guy 1、2、3和5识别SA I/II上与血清型多糖不同的碳水化合物表位。其他MAb识别SA I/II上的蛋白质表位。
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