Department of Anatomy, Lorestan University of Medical Sciences, Khoramabad, Iran.
Iran J Basic Med Sci. 2013 Aug;16(8):906-9.
OBJECTIVE(S): Being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. The purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day-old mice.
Testes of neonate Balb/c mice were vitrified- thawed under standard condition with or without the addition of 100 μM melatonin to both vitrification and thawing solutions. Subsequently, Vitrified-thawed whole testes were digested under standard condition and spermatogonial stem cells type A were separate in the suspension with CD90.1 (Thy1.1(+)) micro beads. Extraction of RNA and synthesis of cDNA was performed. Expression levels of apoptotic genes (Fas, P53, BCL-2 and BAX) were determined using Real-time PCR. Results : With all genes being expressed, level of expression for Fas was higher and for that of P-53 was lower than the remaining genes. Conclusion : Melatonin may cause apoptosis in cells being damaged under the influence of freezing thawing process. In order to examine the exact effects of melatonin on spermatogonia stem cells apoptosis, additional studies are required.
褪黑素由松果体分泌，能诱导正常细胞增殖，诱导癌细胞凋亡。本研究旨在探讨褪黑素对 6 日龄小鼠冻融睾丸生殖细胞主要成分和凋亡基因表达的影响。
采用标准条件对新生 Balb/c 小鼠的睾丸进行玻璃化冷冻-解冻，在玻璃化和解冻溶液中加入或不加入 100μM 褪黑素。然后，在标准条件下消化冻融的整个睾丸，并用 CD90.1（Thy1.1(+）微珠分离生精干细胞 A 型。进行 RNA 提取和 cDNA 合成。采用实时 PCR 法测定凋亡基因（Fas、P53、BCL-2 和 BAX）的表达水平。结果：所有基因均有表达，Fas 基因的表达水平较高，P53 基因的表达水平较低。结论：褪黑素可能导致冷冻-解冻过程中受损细胞发生凋亡。为了研究褪黑素对精原干细胞凋亡的确切影响，需要进一步研究。
