大鼠腹水肝癌细胞中卫星DNA I的链特异性转录
Strand specific transcription of satellite DNA I in rat ascites hepatoma cells.
作者信息
Miyahara M, Sumiyoshi H, Yamamoto M, Endo H
出版信息
Biochem Biophys Res Commun. 1985 Jul 31;130(2):897-903. doi: 10.1016/0006-291x(85)90501-7.
PMID:2411266
Abstract
Rat satellite DNA I was highly expressed in a rat ascites hepatoma cell line, AH60C. The transcripts were found predominantly in the non-polyadenylated fraction of the nuclear RNA. RNA dot blot assay using strand specific probes revealed a strand-selective transcription of the sequence. The structures of cDNA clones isolated on the basis of their cross-hybridization to satellite DNA I were mainly composed of a tandemly repeated array of this sequence.
摘要
大鼠卫星DNA I在大鼠腹水肝癌细胞系AH60C中高表达。转录本主要存在于核RNA的非聚腺苷酸化部分。使用链特异性探针的RNA斑点印迹分析揭示了该序列的链选择性转录。基于与卫星DNA I的交叉杂交分离的cDNA克隆的结构主要由该序列的串联重复阵列组成。
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