在大鼠肿瘤中大量表达的1.5 kb信使核糖核酸在体外编码一种37千道尔顿的蛋白质。

1.5 kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro.

作者信息

Maehara Y, Fujiyoshi T, Takahashi K, Yamamoto M, Endo H

出版信息

Biochem Biophys Res Commun. 1985 Sep 16;131(2):800-5. doi: 10.1016/0006-291x(85)91310-5.

Abstract

A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3' portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.

摘要

对一个与在各种大鼠肿瘤中大量存在的1.5 kb mRNA相对应的cDNA克隆pAH34进行了蛋白质编码能力检测。从大鼠腹水肝癌细胞系AH60C和正常肝脏的多聚腺苷酸加尾（poly(A)+）RNA中经杂交选择的RNA在体外产生了一种37千道尔顿的多肽，但在AH60C中的水平比正常大鼠肝脏中的水平高得多。翻译产物的双向电泳显示该蛋白质的等电点为7.1。对pAH34的核苷酸序列分析表明，该克隆的插入片段由462 bp组成，包含mRNA的3'部分，包括带有位于上游16个核苷酸处居中的AATAAA信号序列的多聚腺苷酸序列、一个短的非翻译区和一个对应于可能67个氨基酸的C末端部分的开放阅读框。