Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
Arch Oral Biol. 2013 Nov;58(11):1594-602. doi: 10.1016/j.archoralbio.2013.08.006. Epub 2013 Aug 22.
Autoinducer 2 (AI-2) is a quorum sensing molecule and plays an important role in dental biofilm formation, mediating interspecies communication and virulence expression of oral bacteria. Fusobacterium nucleatum connects early colonizing commensals and late colonizing periodontopathogens. F. nucleatum AI-2 and quorum sensing inhibitors (QSIs) can manipulate dental biofilm formation. In this study, we evaluated the effect of F. nucleatum AI-2 and QSIs on biofilm formation of Streptococcus gordonii and Streptococcus oralis, which are initial colonizers in dental biofilm. F. nucleatum AI-2 significantly enhanced biofilm growth of S. gordonii and attachment of F. nucleatum to preformed S. gordonii biofilms. By contrast, F. nucleatum AI-2 reduced biofilm growth of S. oralis and attachment of F. nucleatum to preformed S. oralis biofilms. The QSIs, (5Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone and d-ribose, reversed the stimulatory and inhibitory effects of AI-2 on S. gordonii and S. oralis, respectively. In addition, co-culture using a two-compartment system showed that secreted molecules of F. nucleatum had the same effect on biofilm growth of the streptococci as AI-2. Our results demonstrate that early colonizing bacteria can influence the accretion of F. nucleatum, a secondary colonizer, which ultimately influences the binding of periodontopathogens.
自诱导物 2 (AI-2) 是一种群体感应分子，在牙菌斑形成中起着重要作用，介导口腔细菌的种间通讯和毒力表达。核梭杆菌连接早期定植的共生菌和晚期定植的牙周病原菌。核梭杆菌 AI-2 和群体感应抑制剂 (QSIs) 可以操纵牙菌斑的形成。在这项研究中，我们评估了核梭杆菌 AI-2 和 QSIs 对初始定植于牙菌斑的链球菌和口腔链球菌生物膜形成的影响。核梭杆菌 AI-2 显著增强了 S. gordonii 的生物膜生长和核梭杆菌对预先形成的 S. gordonii 生物膜的附着。相比之下，核梭杆菌 AI-2 减少了 S. oralis 的生物膜生长和核梭杆菌对预先形成的 S. oralis 生物膜的附着。QSIs，(5Z)-4-溴-5-(溴亚甲基)-2(5H)-呋喃酮和 D-核糖，分别逆转了 AI-2 对 S. gordonii 和 S. oralis 的刺激和抑制作用。此外，使用双室系统的共培养表明，核梭杆菌分泌的分子对链球菌生物膜生长的影响与 AI-2 相同。我们的结果表明，早期定植的细菌可以影响核梭杆菌的聚集，而核梭杆菌是一种继发性定植菌，最终影响牙周病原菌的结合。
