对艾氏腹水瘤细胞核仁中转录位点的重新评估。

Re-evaluation of the site of transcription in Ehrlich tumour cell nucleoli.

作者信息

Thiry M, Lepoint A, Goessens G

出版信息

Biol Cell. 1985;54(1):57-64. doi: 10.1111/j.1768-322x.1985.tb00380.x.

DOI:10.1111/j.1768-322x.1985.tb00380.x

Abstract

Recent electron microscopic immunolocalization studies (Scheer and Rose, 1984) indicated that RNA polymerase I is located in, and probably confined to, the fibrillar centres of the nucleoli. This conclusion appears to be in contradiction to previous autoradiographic studies reporting that transcription of rRNA genes takes place in the dense fibrillar component. Despite the fact that rRNA transcription occurs in the dense fibrillar component of Ehrlich tumour cell nucleoli, we show in the present paper that dense fibrillar component containing rapidly labelled RNA can be visualized not only around but also inside the fibrillar centres.

摘要

最近的电子显微镜免疫定位研究（舍尔和罗斯，1984年）表明，RNA聚合酶I位于核仁的纤维中心，并且可能局限于此。这一结论似乎与先前的放射自显影研究相矛盾，后者报道rRNA基因的转录发生在致密纤维组分中。尽管埃利希肿瘤细胞核仁的致密纤维组分中发生rRNA转录，但我们在本文中表明，含有快速标记RNA的致密纤维组分不仅可以在纤维中心周围，而且可以在其内部看到。