Thiry M
Institut d'Histologie, Université de Liège, Belgium.
Histochemistry. 1988;89(3):231-6. doi: 10.1007/BF00493145.
The RNA distribution in Ehrlich tumour cell nucleoli has been investigated using RNase-gold method. This technique has been applied to sections of cells prepared under various fixation and embedding conditions. As expected, the specificity and intensity of labelling by gold particles have varied according to the experimental conditions used. Interestingly, however, it has been noted that the localization of gold particles does also vary and in particular within the fibrillar centre. This observation underlines the interest of assaying the RNase-gold complex under various conditions. The gold particles were particularly concentrated over the granular component and to a lesser extent, in the dense fibrillar component. In the latter constituent, it has been noted that the gold markers were preferentially localized at the edge of the dense fibrils. Surprisingly, a few gold particles have also been detected in the fibrillar centres. The weak labelling has persisted even after pepsin or DNase extraction but has completely disappeared after RNase extraction. Further, an inhibition of rRNA synthesis by a treatment with actinomycin D has not produced a significant decrease of the number of gold particles present in the fibrillar centre. These results suggest that fibrillar centres contain a small amount of RNA which would not correspond to pre-rRNA.
利用核糖核酸酶-金标法研究了艾氏瘤细胞核仁中的RNA分布。该技术已应用于在各种固定和包埋条件下制备的细胞切片。正如预期的那样，金颗粒标记的特异性和强度根据所使用的实验条件而有所不同。然而，有趣的是，已经注意到金颗粒的定位也有所不同，特别是在纤维中心内。这一观察结果突出了在各种条件下检测核糖核酸酶-金复合物的重要性。金颗粒特别集中在颗粒成分上，在致密纤维成分中则较少。在后者的成分中，已经注意到金标记物优先定位在致密纤维的边缘。令人惊讶的是，在纤维中心也检测到了一些金颗粒。即使在胃蛋白酶或脱氧核糖核酸酶提取后，弱标记仍然存在，但在核糖核酸酶提取后完全消失。此外，用放线菌素D处理抑制rRNA合成并没有使纤维中心存在的金颗粒数量显著减少。这些结果表明，纤维中心含有少量RNA，这些RNA与前体rRNA不符。
