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Transcription signals in embryonic Xenopus laevis U1 RNA genes.

作者信息

Ciliberto G, Buckland R, Cortese R, Philipson L

出版信息

EMBO J. 1985 Jun;4(6):1537-43. doi: 10.1002/j.1460-2075.1985.tb03814.x.

DOI:10.1002/j.1460-2075.1985.tb03814.x
PMID:2411541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554379/
Abstract

A genomic clone of the most abundant U1 RNA genes from Xenopus laevis was isolated from erythrocyte DNA and sequenced. Two different U1 RNA genes, U1A and U1B, are encoded in an HindIII 1.5-kb fragment and both are expressed after microinjection in Xenopus oocytes. Deletions and site-directed mutagenesis of the clones revealed two promoter elements in the U1B gene; one, located 250-220 nucleotides upstream from the 5' terminus of mature U1 RNA, functions as an activator, yielding a 10-fold promotion of transcription; the other, located 60-50 nucleotides upstream of the cap site, functions as an essential element for promotion of transcription. The U1A gene contained only the latter element in the cloned fragment. Homologous sequences can be identified in several U RNA genes of X. laevis.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/ced1b0eb3777/emboj00271-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/e8fabed67940/emboj00271-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/584c7709b3ac/emboj00271-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/f44b0d809645/emboj00271-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/ced1b0eb3777/emboj00271-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/e8fabed67940/emboj00271-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/584c7709b3ac/emboj00271-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/f44b0d809645/emboj00271-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72aa/554379/ced1b0eb3777/emboj00271-0176-a.jpg

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Transcription signals in embryonic Xenopus laevis U1 RNA genes.
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2
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引用本文的文献

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Transcription of satellite 2 DNA from the newt is driven by a snRNA type of promoter.

本文引用的文献

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The RNA lariat: a new ring to the splicing of mRNA precursors.RNA套索：mRNA前体剪接的新环化形式
Cell. 1984 Dec;39(3 Pt 2):423-5. doi: 10.1016/0092-8674(84)90449-5.
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New M13 vectors for cloning.用于克隆的新型M13载体。
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U1 precursors: variant 3' flanking sequences are transcribed in human cells.U1前体：变异的3'侧翼序列在人类细胞中被转录。
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Only two of the four sites of interaction with nuclear factors within the Xenopus U2 gene promoter are necessary for efficient transcription.非洲爪蟾U2基因启动子内与核因子相互作用的四个位点中，只有两个位点对于高效转录是必需的。
Nucleic Acids Res. 1987 Aug 25;15(16):6437-53. doi: 10.1093/nar/15.16.6437.
9
Identification of proteins interacting with the enhancer of human U2 small nuclear RNA genes.与人类U2小核RNA基因增强子相互作用的蛋白质的鉴定
Nucleic Acids Res. 1987 Jul 10;15(13):4997-5016. doi: 10.1093/nar/15.13.4997.
10
Sequences closely related to an immunoglobulin gene promoter/enhancer element occur also upstream of other eukaryotic and of prokaryotic genes.与免疫球蛋白基因启动子/增强子元件密切相关的序列也存在于其他真核基因和原核基因的上游。
Nucleic Acids Res. 1986 Nov 25;14(22):8819-27. doi: 10.1093/nar/14.22.8819.
Science. 1980 Sep 19;209(4463):1406-14. doi: 10.1126/science.6251548.
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Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.克隆至M13载体的DNA片段的寡核苷酸定向诱变
Methods Enzymol. 1983;100:468-500. doi: 10.1016/0076-6879(83)00074-9.
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Characterization of three chicken pseudogenes for U1 RNA.鸡U1 RNA三个假基因的特征分析
DNA. 1984 Aug;3(4):281-6. doi: 10.1089/dna.1.1984.3.281.
6
Differential expression of multiple U1 small nuclear RNAs in oocytes and embryos of Xenopus laevis.非洲爪蟾卵母细胞和胚胎中多种U1小核RNA的差异表达
Cell. 1984 Oct;38(3):681-9. doi: 10.1016/0092-8674(84)90263-0.
7
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.人U1 RNA的合成。II. 对转录起始于+1位置所必需的启动子两个区域的鉴定。
J Biol Chem. 1984 Jul 10;259(13):8345-52.
8
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.非洲爪蟾U1小核RNA基因：转录活性基因的特征揭示了主要和次要的重复基因家族。
EMBO J. 1984 May;3(5):1075-81. doi: 10.1002/j.1460-2075.1984.tb01931.x.
9
True genes for human U1 small nuclear RNA. Copy number, polymorphism, and methylation.人类U1小核RNA的真实基因。拷贝数、多态性和甲基化。
J Biol Chem. 1984 Feb 10;259(3):2013-21.
10
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.非洲爪蟾U2小核RNA基因：串联重复转录单位，与其他RNA聚合酶II转录基因共享5'和3'侧翼同源性。
EMBO J. 1983;2(11):1883-91. doi: 10.1002/j.1460-2075.1983.tb01675.x.