College of Engineering, Peking University, Beijing, China.
PLoS One. 2013 Oct 7;8(10):e74957. doi: 10.1371/journal.pone.0074957. eCollection 2013.
Keeping mammalian gastrointestinal (GI) tract communities in balance is crucial for host health maintenance. However, our understanding of microbial communities in the GI tract is still very limited. In this study, samples taken from the GI tracts of C57BL/6 mice were subjected to 16S rRNA gene sequence-based analysis to examine the characteristic bacterial communities along the mouse GI tract, including those present in the stomach, duodenum, jejunum, ileum, cecum, colon and feces. Further analyses of the 283,234 valid sequences obtained from pyrosequencing revealed that the gastric, duodenal, large intestinal and fecal samples had higher phylogenetic diversity than the jejunum and ileum samples did. The microbial communities found in the small intestine and stomach were different from those seen in the large intestine and fecal samples. A greater proportion of Lactobacillaceae were found in the stomach and small intestine, while a larger proportion of anaerobes such as Bacteroidaceae, Prevotellaceae, Rikenellaceae, Lachnospiraceae, and Ruminococcaceae were found in the large intestine and feces. In addition, inter-mouse variations of microbiota were observed between the large intestinal and fecal samples, which were much smaller than those between the gastric and small intestinal samples. As far as we can ascertain, ours is the first study to systematically characterize bacterial communities from the GI tracts of C57BL/6 mice.
维持哺乳动物胃肠道（GI）群落的平衡对于宿主健康维护至关重要。然而，我们对胃肠道微生物群落的了解仍然非常有限。在这项研究中，从 C57BL/6 小鼠的胃肠道中采集样本，进行 16S rRNA 基因序列分析，以检查沿小鼠胃肠道存在的特征性细菌群落，包括存在于胃、十二指肠、空肠、回肠、盲肠、结肠和粪便中的细菌群落。对从焦磷酸测序获得的 283,234 个有效序列进行的进一步分析表明，胃、十二指肠、大肠和粪便样本的系统发育多样性高于空肠和回肠样本。小肠和胃中的微生物群落与大肠和粪便样本中的微生物群落不同。胃和小肠中发现了更多的乳杆菌科，而大肠和粪便中则发现了更多的厌氧菌，如拟杆菌科、普雷沃氏菌科、瘤胃球菌科、毛螺菌科和真杆菌科。此外，还观察到大肠和粪便样本之间的微生物群之间存在小鼠间变异，其变异程度远小于胃和小肠样本之间的变异程度。据我们所知，我们的研究是首次系统地描述 C57BL/6 小鼠胃肠道细菌群落的研究。
