Hennies H H
Z Naturforsch C Biosci. 1975 May-Jun;30(3):359-62.
A sulfite reductase from spinach has been purified 125 fold. Throughout all stages of purification the reduction of sulfite has been found dependent on ferredoxin. Reduced ferredoxin has been provided either by photosynthetic reduction in isolated, broken chloroplasts or by NADPH via the ferredoxin-NADP-oxidoreductase. During the purification procedure ferredoxin as electrondonor has been replaced by reduced methylviologen.
一种来自菠菜的亚硫酸盐还原酶已被纯化了125倍。在纯化的各个阶段,均发现亚硫酸盐的还原依赖于铁氧还蛋白。还原型铁氧还蛋白可通过分离破碎的叶绿体中的光合还原作用提供,也可通过铁氧还蛋白-NADP氧化还原酶由NADPH提供。在纯化过程中,作为电子供体的铁氧还蛋白已被还原型甲基紫精所取代。