Esche Rebecca, Scholz Birgit, Engel Karl-Heinz
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München , Maximus-von-Imhof-Forum 2, D-85350 Freising, Germany.
J Agric Food Chem. 2013 Nov 20;61(46):10932-9. doi: 10.1021/jf403046z. Epub 2013 Nov 7.
The suitability of online liquid chromatography-gas chromatography for the analysis of free sterols/stanols, steryl/stanyl fatty acid esters, and trans-steryl/stanyl ferulic acid esters in cereals is demonstrated. The silylated lipid extracts were fractionated via liquid chromatography on a normal phase, and the fractions containing the sterol classes were transferred online to the gas chromatograph for the analysis of their individual compositions. The study provides for the first time data on free sterols/stanols and intact steryl/stanyl esters in sweet corn, popcorn, and proso millet. Sweet corn revealed the highest contents of free sterols/stanols and steryl/stanyl fatty acid esters, and popcorn, in turn, the highest amounts of trans-steryl/stanyl ferulic acid esters. The distribution patterns of the proso millet samples revealed pronounced differences from those of sweet corn and popcorn as they particularly exhibited high proportions of free cholesterol and cholesteryl fatty acid esters. Furthermore, no trans-steryl/stanyl ferulic acid esters could be detected.
本文证明了在线液相色谱 - 气相色谱法用于分析谷物中游离甾醇/甾烷醇、甾醇/甾烷醇脂肪酸酯和反式甾醇/甾烷醇阿魏酸酯的适用性。硅烷化脂质提取物通过正相液相色谱进行分离，含有甾醇类的馏分在线转移至气相色谱仪以分析其各自的组成。该研究首次提供了甜玉米、爆米花和黍中游离甾醇/甾烷醇以及完整甾醇/甾烷醇酯的数据。甜玉米中游离甾醇/甾烷醇和甾醇/甾烷醇脂肪酸酯的含量最高，而爆米花中反式甾醇/甾烷醇阿魏酸酯的含量最高。黍样品的分布模式与甜玉米和爆米花明显不同，因为它们特别表现出游离胆固醇和胆固醇脂肪酸酯的高比例。此外，未检测到反式甾醇/甾烷醇阿魏酸酯。
