López-Cobo Ana, Martín-García Beatriz, Segura-Carretero Antonio, Fernández-Gutiérrez Alberto, Gómez-Caravaca Ana María
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071 Granada, Spain.
Functional Food Research and Development Center, Health Science Technological Park, Avd. del Conocimiento, Bioregion Building, 18100 Granada, Spain.
Int J Mol Sci. 2017 Jul 22;18(7):1594. doi: 10.3390/ijms18071594.
Two different gas chromatography coupled to quadrupole-time of flight mass spectrometry (GC-QTOF-MS) methodologies were carried out for the analysis of phytosterols and tocopherols in the flesh of three mango cultivars and their by-products (pulp, peel, and seed). To that end, a non-polar column ((5%-phenyl)-methylpolysiloxane (HP-5ms)) and a mid-polar column (crossbond trifluoropropylmethyl polysiloxane (RTX-200MS)) were used. The analysis time for RTX-200MS was much lower than the one obtained with HP-5ms. Furthermore, the optimized method for the RTX-200MS column had a higher sensibility and precision of peak area than the HP-5ms methodology. However, RTX-200MS produced an overlapping between β-sitosterol and Δ⁵-avenasterol. Four phytosterols and two tocopherols were identified in mango samples. As far as we are concerned, this is the first time that phytosterols have been studied in mango peel and that Δ⁵-avenasterol has been reported in mango pulp. α- and γ-tocopherol were determined in peel, and α-tocopherol was the major tocopherol in this fraction (up to 81.2%); however, only α-tocopherol was determined in the pulp and seed. The peel was the fraction with the highest total concentration of phytosterols followed by seed and pulp, and "Sensación" was the cultivar with the highest concentration of total phytosterols in most cases. There were no significant differences between quantification of tocopherols with both columns. However, in most cases, quantification of phytosterols was higher with RTX-200MS than with HP-5ms column.
采用两种不同的气相色谱-四极杆飞行时间质谱联用（GC-QTOF-MS）方法，对三个芒果品种及其副产物（果肉、果皮和种子）中的植物甾醇和生育酚进行分析。为此，使用了一根非极性柱（（5%-苯基）-甲基聚硅氧烷（HP-5ms））和一根中极性柱（交联三氟丙基甲基聚硅氧烷（RTX-200MS））。RTX-200MS的分析时间远低于HP-5ms。此外，RTX-200MS柱的优化方法比HP-5ms方法具有更高的灵敏度和峰面积精密度。然而，RTX-200MS会使β-谷甾醇和Δ⁵-燕麦甾醇之间出现重叠。在芒果样品中鉴定出了四种植物甾醇和两种生育酚。就我们所知，这是首次对芒果果皮中的植物甾醇进行研究，也是首次在芒果果肉中报道Δ⁵-燕麦甾醇。在果皮中测定了α-和γ-生育酚，α-生育酚是该组分中的主要生育酚（高达81.2%）；然而，在果肉和种子中仅测定出了α-生育酚。果皮是植物甾醇总浓度最高的部分，其次是种子和果肉，在大多数情况下，“Sensación”品种的植物甾醇总浓度最高。两种柱子对生育酚的定量分析没有显著差异。然而，在大多数情况下，RTX-200MS对植物甾醇的定量分析结果高于HP-5ms柱。
