城市家禽群中李斯特菌的爆发。
Outbreak of Listeria monocytogenes in an urban poultry flock.
机构信息
Avian Health and Food Safety Laboratory, Washington Animal Disease Diagnostic Laboratory, Washington State University, 2607 West Pioneer, Puyallup, WA 98371, USA.
出版信息
BMC Vet Res. 2013 Oct 11;9:204. doi: 10.1186/1746-6148-9-204.
BACKGROUND
Listeria monocytogenes infection is most commonly recognized in ruminants, including cattle, sheep, and goats; but it is rarely diagnosed in poultry. This report describes an outbreak of L. monocytogenes in a backyard poultry flock. Also, it points out the importance of collaboration between veterinarians and public health departments and the possible implications of zoonotic diseases.
CASE PRESENTATION
Depression, lack of appetite, labored breathing, and increased mortality were noted for 5 months in several affected birds within the flock. The pathologic changes in the internal organs of infected birds included severe myocarditis, pericarditis, pneumonia, hepatitis, and splenitis. No lesions were noted in the brain. Gram-positive organisms were seen in histologic sections of the heart and spleen. Listeria monocytogenes was detected by real time PCR from formalin fixed heart and spleen, and was isolated from fresh lung, spleen, and liver. This isolate was identified as L. monocytogenes serotype 4b by 16S rDNA sequencing and by PCR-based serotyping assay.
CONCLUSIONS
This is the first report describing outbreak of L. monocytogenes in backyard poultry flock in Washington State and use of molecular methods to confirm L. monocytogenes infection from formalin fixed tissues.
背景
单核细胞增生李斯特菌感染最常发生在反刍动物中，包括牛、羊和山羊；但在禽类中很少被诊断出来。本报告描述了后院家禽群中单核细胞增生李斯特菌的暴发。它还指出了兽医和公共卫生部门之间合作的重要性以及人畜共患病的可能影响。
病例介绍
在该禽群中，数只患病禽只出现了 5 个月的抑郁、食欲不振、呼吸困难和死亡率增加的症状。受感染禽只的内脏器官病变包括严重心肌炎、心包炎、肺炎、肝炎和脾炎。大脑无病变。心脏和脾脏的组织学切片中可见革兰氏阳性菌。实时 PCR 从福尔马林固定的心脏和脾脏中检测到李斯特菌单核细胞增生症，从新鲜的肺、脾和肝中分离出该菌。通过 16S rDNA 测序和基于 PCR 的血清型鉴定试验，该分离株被鉴定为单核细胞增生李斯特菌血清型 4b。
结论
这是华盛顿州首例描述后院家禽群中单核细胞增生李斯特菌暴发的报告，并使用分子方法从福尔马林固定组织中确认李斯特菌单核细胞增生症感染。
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