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甘蓝型油菜 BrCRTISO1 基因衍生的分子标记与富含脯氨酸的橙色叶片的关联[已更正]。

Association of molecular markers derived from the BrCRTISO1 gene with prolycopene-enriched orange-colored leaves in Brassica rapa [corrected].

出版信息

Theor Appl Genet. 2014 Jan;127(1):179-91. doi: 10.1007/s00122-013-2209-3.

Abstract

Sequence polymorphism in BrCRTISO1, encoding carotenoid isomerase, is identified in orange-colored B. rapa , and three resulting gene-based markers will be useful for marker-assisted breeding of OC cultivars. Carotenoids are color pigments that are important for protection against excess light in plants and essential sources of retinols and vitamin A for animals. We identified a single recessive gene that might cause orange-colored (OC) inner leaves in Brassica rapa. The inner leaves of the OC cultivar were enriched in lycopene-like compounds, specifically prolycopene and its isomers, which can be a useful functional trait for Kimchi cabbage. We used a candidate gene approach based on the 21 genes in the carotenoid pathway to identify a candidate gene responsible for the orange color. Among them, we focused on two carotenoid isomerase (CRTISO) genes, BrCRTISO1 and BrCRTISO2. The expression of BrCRTISO1 was higher than that of BrCRTISO2 in a normal yellow-colored (YE) cultivar, but full-length BrCRTISO1 transcripts were not detected in the OC cultivar. Genomic sequence analysis revealed that BrCRTISO1 of the OC cultivar had many sequence variations, including single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels), compared to that of the YE cultivar. We developed molecular makers for the identification of OC phenotype based on the polymorphic regions within BrCRTISO1 in B. rapa breeding. The BrCRTISO1 gene and its markers identified in this study are novel genetic resources and will be useful for studying the carotenoid biosynthesis pathway as well as developing new cultivars with unique carotenoid contents in Brassica species.

摘要

序列多态性 BrCRTISO1 ,编码类胡萝卜素异构酶,在橙色的 B. rapa 中被鉴定出来,三个由此产生的基于基因的标记将有助于 OC 品种的标记辅助育种。类胡萝卜素是一种重要的色素,对植物的过量光照有保护作用,也是动物维生素 A 和视黄醇的重要来源。我们鉴定出一个可能导致 B. rapa 内叶呈橙色(OC)的隐性单基因。OC 品种的内叶富含番茄红素样化合物,特别是脯氨酸及其异构体,这对泡菜白菜来说是一个有用的功能性状。我们基于类胡萝卜素途径中的 21 个基因,采用候选基因方法,鉴定出一个可能导致橙色的候选基因。在这些基因中,我们重点关注两个类胡萝卜素异构酶(CRTISO)基因,BrCRTISO1 和 BrCRTISO2。在正常的黄色(YE)品种中,BrCRTISO1 的表达高于 BrCRTISO2,但在 OC 品种中未检测到全长 BrCRTISO1 转录本。基因组序列分析显示,与 YE 品种相比,OC 品种的 BrCRTISO1 有许多序列变异,包括单核苷酸多态性(SNP)和插入和缺失(InDels)。我们根据 B. rapa 育种中 BrCRTISO1 的多态性区域,开发了用于鉴定 OC 表型的分子标记。本研究中鉴定的 BrCRTISO1 基因及其标记是新的遗传资源,将有助于研究类胡萝卜素生物合成途径以及开发具有独特类胡萝卜素含量的 Brassica 品种。

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