Scheynius A, Tjernlund U
Scand J Immunol. 1985 Aug;22(2):227-33. doi: 10.1111/j.1365-3083.1985.tb01875.x.
The presence of class II transplantation antigens on keratinocytes and the phenotypes of the inflammatory cells in the late human skin tuberculin reaction were analysed with immunohistochemical double staining techniques in frozen sections of skin biopsies taken 10-45 days after intradermal purified protein derivative (PPD) injection. Dermal cell infiltrates decreased with time but were found throughout the observation period. Most of the cells in the perivascular infiltrates expressed HLA-DR antigens. Some of these cells are probably 'activated' macrophages, since they expressed OKM1 and OKT9 antigens in consecutive sections. Another less frequent cell population which reacted with RFD1 antibodies are presumably interdigitating cells. About half the perivascular cells were anti-Leu 3a-positive (T 'helper/inducer' phenotype). HLA-DR but not HLA-DQ antigens were detected on keratinocytes. This acquired expression of HLA-DR antigens on the epithelial cells disappeared between 17 and 30 days. If HLA-DR-expressing keratinocytes have immunoregulatory functions these might be different from those of other HLA-DR-expressing cell types that also express HLA-DQ molecules.
采用免疫组化双重染色技术，对皮内注射纯化蛋白衍生物（PPD）10 - 45天后所取皮肤活检组织冰冻切片中的角质形成细胞上II类移植抗原的存在情况以及人类皮肤结核菌素反应后期炎症细胞的表型进行了分析。真皮细胞浸润随时间减少，但在整个观察期内均能发现。血管周围浸润中的大多数细胞表达HLA - DR抗原。其中一些细胞可能是“活化的”巨噬细胞，因为它们在连续切片中表达OKM1和OKT9抗原。另一个与RFD1抗体反应的较少见细胞群可能是交错突细胞。约一半的血管周围细胞抗Leu 3a呈阳性（T“辅助/诱导”表型）。在角质形成细胞上检测到HLA - DR抗原，但未检测到HLA - DQ抗原。上皮细胞上这种HLA - DR抗原的获得性表达在17至30天之间消失。如果表达HLA - DR的角质形成细胞具有免疫调节功能，那么这些功能可能与其他也表达HLA - DQ分子的表达HLA - DR的细胞类型不同。
