Caines Deanne, Sinclair Melissa, Wood Darren, Valverde Alexander, Dyson Doris, Gaitero Luis, Nykamp Stephanie
Department of Clinical Studies (Caines, Sinclair, Valverde, Dyson, Gaitero, Nykamp) and Department of Pathobiology (Wood), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
Can J Vet Res. 2013 Oct;77(4):297-302.
The objectives of this study were to establish a reference interval for canine cerebrospinal fluid lactate (CSFL) and to compare CSFL and plasma lactate (PL) concentrations in anesthetized dogs with and without intracranial disease. Using a prospective study, canine blood and cerebrospinal fluid were collected for lactate analysis in 11 dogs with intracranial disease after undergoing magnetic resonance imaging (MRI) (Group ID-MRI), in 10 healthy dogs post-MRI (Group H-MRI), and in 39 healthy dogs after induction of anesthesia (Group H-Sx). Dogs were anesthetized for the procedures using different anesthetic protocols. Neurological scores (NS) and sedation scores (SS) were assessed pre-anesthesia in ID-MRI dogs. The CSFL reference interval [90% confidence interval (CI) for lower and upper limits] was 1.1 (1.0 to 1.2) to 2.0 (2.0 to 2.1) mmol/L. Mean ± SD CSFL concentrations were: ID-MRI, 2.1 ± 0.8; H-MRI, 1.6 ± 0.4; and H-Sx, 1.6 ± 0.2 mmol/L. There was a tendency for higher CSFL in dogs in the ID-MRI group than in those in the H-MRI or H-Sx groups (P = 0.12). There was agreement between CSFL and PL in ID-MRI dogs (P = 0.007), but not in dogs in H-MRI (P = 0.5) or H-Sx (P = 0.2). Of the ID-MRI dogs, those with worse NS had higher CSFL (r (2) = 0.44). The correlation between CSFL and PL in dogs with intracranial disease and between worse NS and higher CSFL warrants further investigation into the use of CSFL and PL for diagnostic and prognostic purposes.
本研究的目的是建立犬脑脊液乳酸（CSFL）的参考区间，并比较患有和未患有颅内疾病的麻醉犬的CSFL和血浆乳酸（PL）浓度。采用前瞻性研究，在11只患有颅内疾病的犬接受磁共振成像（MRI）后（ID-MRI组）、10只健康犬MRI后（H-MRI组）以及39只麻醉诱导后的健康犬（H-Sx组）中采集犬的血液和脑脊液进行乳酸分析。使用不同的麻醉方案对犬进行麻醉以实施这些操作。在ID-MRI组的犬麻醉前评估神经学评分（NS）和镇静评分（SS）。CSFL参考区间[下限和上限的90%置信区间（CI）]为1.1（1.0至1.2）至2.0（2.0至2.1）mmol/L。CSFL平均浓度±标准差分别为：ID-MRI组，2.1±0.8；H-MRI组，1.6±0.4；H-Sx组，1.6±0.2 mmol/L。ID-MRI组犬的CSFL有高于H-MRI组或H-Sx组犬的趋势（P = 0.12）。ID-MRI组犬的CSFL和PL之间存在一致性（P = 0.007），但H-MRI组犬（P = 0.5）或H-Sx组犬（P = 0.2）不存在。在ID-MRI组犬中，NS较差的犬CSFL较高（r (2) = 0.44）。颅内疾病犬的CSFL和PL之间以及较差的NS和较高的CSFL之间的相关性值得进一步研究CSFL和PL在诊断和预后方面的应用。
