与老式高效液相色谱法相比，采用新型爱科来高效液相色谱法测定糖化血红蛋白（HbA1c）时，变异血红蛋白中HbA1c与血糖之间的差异程度更小。

Degree of discrepancy between HbA1c and glycemia in variant hemoglobin is smaller when HbA1c is measured by new-type Arkray HPLC compared with old-type HPLC.

作者信息

Koga Masafumi, Kurebayashi Shogo, Murai Jun, Saito Hiroshi, Miyazaki Ayako

机构信息

Department of Internal Medicine, Kawanishi City Hospital, Hyogo, Japan.

Department of Internal Medicine, Nishinomiya Municipal Hospital, Hyogo, Japan.

出版信息

Clin Biochem. 2014 Jan;47(1-2):123-5. doi: 10.1016/j.clinbiochem.2013.09.019. Epub 2013 Oct 12.

DOI:10.1016/j.clinbiochem.2013.09.019

PMID:24128409

Abstract

OBJECTIVES

Although variant hemoglobin mainly demonstrates inappropriate HbA1c values measured by high-performance liquid chromatography (HPLC), these values differ depending on the HPLC model. In the 1990s, old-type HPLC models were replaced with new-type HPLC models which could separate stable HbA1c from labile HbA1c and modified hemoglobin. This study compared HbA1c values in subjects with variant hemoglobin measured using old-type Arkray HPLC (HA-8150) and new-type Arkray HPLC (HA-8160 or HA-8180).

DESIGN AND METHODS

This study included non-diabetic subjects with apparently low HbA1c values who had variant hemoglobins due to a β-chain heterozygous mutation. HbA1c was measured by old-type HPLC in 28 subjects with 12 variant hemoglobins (group 1) and new-type HPLC in six subjects with four variant hemoglobins (group 2). When HbA1c was measured by HPLC (HPLC-HbA1c), HbA1c measured by immunoassay (IA-HbA1c) and glycated albumin (GA) were also measured.

RESULTS

IA-HbA1c and GA did not significantly differ between both groups. However, HPLC-HbA1c in group 2 was significantly higher than that in group 1 (group 1: 2.9 ± 0.7% vs. group 2: 3.7 ± 0.2%, P = 0.006).

CONCLUSIONS

When HbA1c in subjects with variant hemoglobin is measured by new-type Arkray HPLC, the degree of discrepancy between HbA1c and glycemia is smaller compared with that measured by old-type HPLC.

摘要

目的

尽管变异血红蛋白主要表现为通过高效液相色谱法（HPLC）测得的糖化血红蛋白（HbA1c）值不准确，但这些值会因HPLC型号的不同而有所差异。在20世纪90年代，旧型号的HPLC被新型HPLC所取代，新型HPLC能够将稳定的HbA1c与不稳定的HbA1c及修饰血红蛋白分离。本研究比较了使用旧型号爱科来HPLC（HA - 8150）和新型爱科来HPLC（HA - 8160或HA - 8180）测定的变异血红蛋白患者的HbA1c值。

设计与方法

本研究纳入了因β链杂合突变而患有变异血红蛋白、糖化血红蛋白值明显较低的非糖尿病患者。对28例患有12种变异血红蛋白的患者采用旧型号HPLC测定HbA1c（第1组），对6例患有4种变异血红蛋白的患者采用新型HPLC测定HbA1c（第2组）。当通过HPLC测定HbA1c（HPLC - HbA1c）时，还测定了通过免疫测定法测得的HbA1c（IA - HbA1c）和糖化白蛋白（GA）。