Dong Yihui, Lin Weifeng, Laaksonen Aatto, Ji Xiaoyan
Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Rehovot 76100, Israel.
Energy Engineering, Division of Energy Science, Luleå University of Technology, 97187 Luleå, Sweden.
Membranes (Basel). 2022 Apr 11;12(4):415. doi: 10.3390/membranes12040415.
Understanding the adsorption and interaction between porous materials and protein is of great importance in biomedical and interface sciences. Among the studied porous materials, TiO and its hybrid materials, featuring distinct, well-defined pore sizes, structural stability and excellent biocompatibility, are widely used. In this review, the use of four powerful, synergetic and complementary techniques to study protein-TiObased porous materials interactions at different scales is summarized, including high-performance liquid chromatography (HPLC), atomic force microscopy (AFM), surface-enhanced Raman scattering (SERS), and Molecular Dynamics (MD) simulations. We expect that this review could be helpful in optimizing the commonly used techniques to characterize the interfacial behavior of protein on porous TiO materials in different applications.
了解多孔材料与蛋白质之间的吸附和相互作用在生物医学和界面科学中具有重要意义。在所研究的多孔材料中，TiO及其杂化材料具有独特、明确的孔径、结构稳定性和优异的生物相容性，被广泛应用。在本综述中，总结了四种强大、协同且互补的技术在不同尺度上研究基于蛋白质-TiO的多孔材料相互作用的应用，包括高效液相色谱（HPLC）、原子力显微镜（AFM）、表面增强拉曼散射（SERS）和分子动力学（MD）模拟。我们期望本综述有助于优化常用技术，以表征蛋白质在不同应用中多孔TiO材料上的界面行为。
