Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Food Chem. 2014 Feb 15;145:306-11. doi: 10.1016/j.foodchem.2013.08.031. Epub 2013 Aug 27.
Five genotypes of Bene were collected from West Azerbaijan, Kurdistan, Kermanshah and Ilam provinces, Iran. Total phenolic and flavonoid contents were determined and antioxidant activity of the extracts were measured using different assays: FRAP, nitric oxide radical scavenging and DPPH. Total phenolic and flavonoid contents of the hull extracts were significantly higher than shell and kernel extracts. In hull extracts, positive correlation coefficient was observed between total phenolic content and FRAP (R(2) = 0.98) and DPPH (R(2) = 0.66) assays. The results showed that, the highest antioxidant activity of hull extract may be attributed to higher total phenolic content. Analyzing the phenolic composition using HPLC, sinapic acid, vanillic acid and p-hydroxybenzoic acid were found in hull and shell extracts of all genotypes. Among all the genotypes studied here, B4 showed the highest phenolic content and antioxidant activity.
从伊朗的西阿塞拜疆省、库尔德斯坦省、克尔曼沙阿省和伊拉姆省收集了 5 种贝尼基因型。测定了总酚和类黄酮含量，并使用不同的测定方法测量了提取物的抗氧化活性：FRAP、一氧化氮自由基清除和 DPPH。壳提取物的总酚和类黄酮含量明显高于壳和核提取物。在壳提取物中，总酚含量与 FRAP（R²=0.98）和 DPPH（R²=0.66）测定之间观察到正相关系数。结果表明，壳提取物具有最高的抗氧化活性，可能归因于其较高的总酚含量。使用 HPLC 分析酚类成分，在所有基因型的壳和壳提取物中均发现了芥子酸、香草酸和对羟基苯甲酸。在所研究的所有基因型中，B4 表现出最高的酚类含量和抗氧化活性。
