Faerman A I, Maksimenko A V, Tonevitsky A G, Ilynsky O B, Torchilin V P
Experientia. 1985 Oct 15;41(10):1342-4. doi: 10.1007/BF01952086.
Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction of cultured cells with the conjugates obtained and with control substances was monitored following incorporation of 14C-leucine radioactivity. It was found that 14C-leucine incorporation was suppressed by 80-90% at a conjugate concentration of 10(-6)-10(-7) M. Antigen-negative cells (line IAR; rat hepatocytes) were insensitive to the conjugate at any concentration used. Control use of purified ricin A-chain, native or oxidized dextran, specific and nonspecific IgG did not affect normal 14C-leucine incorporation. The data obtained may be useful for designing targeted drug transport systems and for selective screening of modulated smooth cells in vascular pathology models in vivo.
最初从大鼠主动脉中膜分离得到的经调节的平滑肌细胞表面抗原的单克隆抗体，通过氧化葡聚糖桥与蓖麻毒素A链偶联。在掺入14C-亮氨酸放射性后，监测培养细胞与所得偶联物以及对照物质的相互作用。结果发现，在偶联物浓度为10(-6)-10(-7)M时，14C-亮氨酸掺入被抑制80-90%。抗原阴性细胞（IAR系；大鼠肝细胞）在所用的任何浓度下对偶联物均不敏感。纯化的蓖麻毒素A链、天然或氧化葡聚糖、特异性和非特异性IgG的对照使用均不影响正常的14C-亮氨酸掺入。所获得的数据可能有助于设计靶向药物输送系统，以及在体内血管病理模型中对经调节的平滑肌细胞进行选择性筛选。
