Faerman A I, Tjurmin A V
Exp Cell Res. 1987 Mar;169(1):85-94. doi: 10.1016/0014-4827(87)90227-8.
Monoclonal antibody L1 has been obtained after immunization of BALB/c mice with long-term cultured smooth muscle cells (SMC) originally isolated from rat aortic media. Antibody L1 recognizes only the surface antigen of cultured SMC and does not react with other cultured rat cell types. It has been shown that in primary culture of SMC the L1-positive cells appear on the 2nd to 3rd day and their proportion increases up to the 7th day up to 40% in DMEM supplemented with 10% of fetal calf serum (FCS), up to 25% in DMEM with 5% of rat whole-blood serum, but up to only 5% in DMEM with 5% rat plasma-derived serum. These results are in agreement with data on [14C]thymidine incorporation and on flow cytometry. Using FACS II, the SMC were sorted into subpopulations on the 4th and 8th days of primary culture according to the intensity of their specific immunofluorescence. It has been found that the DNA profile in intensively labelled cells corresponds to that in an intensively proliferating population of cells. These findings suggest that antigen L1 appears to be the specific marker of modulated SMC entering the cell cycle.
用最初从大鼠主动脉中膜分离并长期培养的平滑肌细胞（SMC）免疫BALB/c小鼠后，获得了单克隆抗体L1。抗体L1仅识别培养的SMC的表面抗原，而不与其他培养的大鼠细胞类型发生反应。结果表明，在SMC原代培养中，L1阳性细胞在第2至3天出现，在补充10%胎牛血清（FCS）的DMEM中，其比例在第7天增加至40%；在含有5%大鼠全血血清的DMEM中，比例增加至25%；但在含有5%大鼠血浆衍生血清的DMEM中，比例仅增加至5%。这些结果与[14C]胸腺嘧啶核苷掺入和流式细胞术的数据一致。在原代培养的第4天和第8天，使用FACS II根据其特异性免疫荧光强度将SMC分选成亚群。已发现，强标记细胞中的DNA图谱与细胞增殖活跃群体中的DNA图谱一致。这些发现表明，抗原L1似乎是进入细胞周期的调节性SMC的特异性标志物。
