Kaur Inderjit, Bhatia Sneh, Bhati Yogendra, Sharma Vinay, Mediratta Pramod K, Bhattacharya Swapan K
J Basic Clin Physiol Pharmacol. 2014 May 1;25(2):211-5. doi: 10.1515/jbcpp-2013-0083.
Murraya koenigii (Rutaceae) (curry patta: Hindi) of the family Rutaceae is used in the traditional Indian system of medicine for its immunomodulatory properties. The essential oil of the leaves of M. koenigii possesses antimicrobial, antifungal, and pesticidal activities and is used for the treatment of amebiasis, diabetes, and hepatitis. The present study was performed to evaluate the effect of M. koenigii on humoral and cell-mediated immune responses in rats.
Aqueous extract of M. koenigii leaves was administered orally in a dose of 350 mg/kg. Cell-mediated immunity was assessed by measuring foot pad thickness following sensitization by injection of keyhole limpet hemocyanin and subsequent challenge by the same. Humoral immunity was assessed by measurement of hemagglutination titer to sheep red blood cells (SRBCs).
In the humoral immune response, the administration of M. koenigii [350 mg/kg per os (p.o.)] from day 1 to day 7 after sensitization with SRBC on day 0 caused a significant increase in the primary anti-SRBC titer. However, the secondary immune response was decreased significantly (p<0.05) as shown by a decrease in secondary anti-SRBC titer measured on day 11 following a booster dose of antigen on day 8. In the delayed-type hypersensitivity test, M. koenigii (350 mg/kg, p.o.), when administered for 14 days, produced a significant (p<0.05) decrease in foot pad thickness when compared with the control group.
Thus, these results suggest that oral administration of M. koenigii augments primary humoral immune response and decreases cell-mediated immunity.
芸香科的印度楝（印地语：咖喱叶）在传统印度医学体系中因其免疫调节特性而被使用。印度楝叶片的精油具有抗菌、抗真菌和杀虫活性，可用于治疗阿米巴病、糖尿病和肝炎。本研究旨在评估印度楝对大鼠体液免疫和细胞介导免疫反应的影响。
以350 mg/kg的剂量口服给予印度楝叶片水提取物。通过注射匙孔血蓝蛋白致敏并随后再次注射相同物质后测量足垫厚度来评估细胞介导的免疫。通过测量对绵羊红细胞（SRBC）的血凝滴度来评估体液免疫。
在体液免疫反应中，在第0天用SRBC致敏后从第1天到第7天口服给予印度楝[350 mg/kg经口（p.o.）]导致初次抗SRBC滴度显著增加。然而，如在第8天给予抗原加强剂量后第11天测量的二次抗SRBC滴度降低所示，二次免疫反应显著降低（p<0.05）。在迟发型超敏反应试验中，与对照组相比，口服给予印度楝（350 mg/kg，p.o.）14天时足垫厚度显著降低（p<0.05）。
因此，这些结果表明口服印度楝可增强初次体液免疫反应并降低细胞介导的免疫。
