Person S, Warner S C, Bzik D J, Debroy C, Fox B A
Gene. 1985;35(3):279-87. doi: 10.1016/0378-1119(85)90006-x.
A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZ alpha expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].
已分离出一种带有编码单纯疱疹病毒2型（HSV - 2）糖蛋白B（gB）基因插入片段的质粒。将编码HSV - 2 gB肽一部分的DNA序列克隆到细菌lacZα表达载体中，并用于转化大肠杆菌。在诱导lacZpo启动的转录后，一些细菌变成丝状并产生含有大量65 kDa肽的包涵体，该肽被证明能被针对HSV - 2和HSV - 1的广谱抗体沉淀。这些克隆之一的HSV - 2插入片段指定了与HSV - 1 gB的135至629位氨基酸残基相对应的氨基酸残基[Bzik等人，《病毒学》133（1984）301 - 314]。
