Amann E, Bröker M, Wurm F
Gene. 1984 Dec;32(1-2):203-15. doi: 10.1016/0378-1119(84)90048-9.
DNA fragments encoding structural information of the Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) gene were cloned into pUC plasmids [Vieira and Messing, Gene 19 (1982) 259-268]. None of the hybrid plasmids were able to direct the synthesis of significant amounts of gC related peptides. Several of the plasmid-bearing strains, however, exhibited inhibition characteristics which can be correlated with the presence on the plasmid of specific gC gene sequences. After insertion of gC DNA fragments into expression vector pMF2 between phage lambda repressor gene cI and lacZ, significant amounts of cI::gC::beta-galactosidase fusion proteins are synthesized. These tripartite fusion proteins are immunologically reactive with anti-HSV-1 antisera. The expression system based on pMF2 can be generally used to identify and express foreign antigens in Escherichia coli.
编码单纯疱疹病毒1型（HSV-1）糖蛋白C（gC）基因结构信息的DNA片段被克隆到pUC质粒中[维埃拉和梅辛，《基因》19（1982年）259 - 268]。没有一个杂交质粒能够指导合成大量与gC相关的肽。然而，一些携带质粒的菌株表现出抑制特性，这可能与质粒上特定gC基因序列的存在有关。将gC DNA片段插入噬菌体λ阻遏基因cI和lacZ之间的表达载体pMF2后，合成了大量的cI::gC::β-半乳糖苷酶融合蛋白。这些三方融合蛋白与抗HSV-1抗血清具有免疫反应性。基于pMF2的表达系统一般可用于在大肠杆菌中鉴定和表达外源抗原。
