Watson R J, Weis J H, Salstrom J S, Enquist L W
Science. 1982 Oct 22;218(4570):381-4. doi: 10.1126/science.6289440.
The protein coding region of the herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.
绘制了单纯疱疹病毒1型糖蛋白D（gD）基因的蛋白质编码区，并测定了核苷酸序列。发现gD多肽的预测氨基酸序列与其他病毒糖蛋白有许多共同特征。将该蛋白质编码区插入细菌表达质粒可使大肠杆菌合成具有免疫反应性的gD相关多肽。讨论了该系统用于制备通用型单纯疱疹病毒疫苗的潜力。
