Badowska-Szalewska Ewa, Ludkiewicz Beata, Sidor-Kaczmarek Justyna, Lietzau Grazyna, Spodnik Jan H, Świetlik Dariusz, Domaradzka-Pytel Beata, Moryś Janusz
Department of Anatomy and Neurobiology,
Acta Neurobiol Exp (Wars). 2013;73(3):364-78. doi: 10.55782/ane-2013-1943.
It is postulated that stress differentially affects interleukin-1beta (IL-1beta) during ontogenetic life. This study examined the influence of chronic exposure to forced swim (FS) stress or high-light open-field (HL-OF) stress on interleukin-1beta (IL-1beta). The total level of IL-1beta protein was assessed by Western blot analysis of hippocampal extracts. Double immunofluorescence staining was used to reveal the percentage of IL-1beta/NeuN (NeuN - neuronal marker) cells in the CA1, CA3 and dentate gyrus (DG) hippocampal subfields. Juvenile (P28; P - postnatal day) and middle-aged (P360) rats were used in the experiment. The research showed no significant differences in IL-1beta protein levels between P28 and P360 non-stress rats. However, a substantial increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG in P360 rats was observed. Chronic FS had no significant influence on IL-1beta expression in the hippocampus or on the percentage of IL-1beta-ir neurons in CA1, CA3 and DG hippocampal subfields in either age group. During HL-OF, the IL-1beta level was significantly increased in the hippocampus of P28 and P360 rats, whereas a marked increase in the percentage of IL-1beta-ir neurons in the CA1, CA3 and DG hippocampal areas occurred only in P360 animals. These results indicate that chronic HL-OF stimulation was the factor inducing changes in the IL-1beta protein levels in P28 and P360 rats and in the percentage of IL-1beta/NeuN-ir cells in the hippocampus of P360 animals.
据推测，应激在个体发育过程中对白细胞介素-1β（IL-1β）有不同影响。本研究检测了慢性强迫游泳（FS）应激或强光旷场（HL-OF）应激对白细胞介素-1β（IL-1β）的影响。通过对海马提取物进行蛋白质免疫印迹分析来评估IL-1β蛋白的总水平。采用双重免疫荧光染色来显示海马CA1、CA3和齿状回（DG）亚区中IL-1β/NeuN（NeuN - 神经元标记物）细胞的百分比。实验使用了幼年（P28；P - 出生后天数）和中年（P360）大鼠。研究表明，P28和P360非应激大鼠的IL-1β蛋白水平无显著差异。然而，观察到P360大鼠CA1、CA3和DG中IL-1β免疫反应性神经元的百分比大幅增加。慢性FS对两个年龄组大鼠海马中IL-1β的表达或CA1、CA3和DG海马亚区中IL-1β免疫反应性神经元的百分比均无显著影响。在HL-OF期间，P28和P360大鼠海马中的IL-1β水平显著升高，而仅在P360动物的CA1、CA3和DG海马区域中IL-1β免疫反应性神经元的百分比显著增加。这些结果表明，慢性HL-OF刺激是诱导P28和P360大鼠IL-1β蛋白水平变化以及P360动物海马中IL-1β/NeuN免疫反应性细胞百分比变化的因素。
