Badowska-Szalewska E, Klejbor I, Sidor-Kaczmarek J, Cecot T, Lietzau G, Spodnik J H, Moryś J
Deaprtment of Anatomy and Neurobiology, Medical University of Gdańsk, Poland.
Folia Morphol (Warsz). 2009 Aug;68(3):119-28.
The aim of this study was to investigate the influence of two periods of life, namely P28 and P360, on the changes in interleukin-1beta (IL-1beta) immunoreactivity (-ir) in the hippocampus (CA1, CA3, DG) and amygdala (central-CeA, medial-MeA) caused by acute and repeated open field (OF), or by forced swim (FS) exposition. Rats were divided into groups: non-stressed, exposed to acute (one-time for 15 min) and chronic stressors (21 days for 15 min daily). We found IL-1beta-ir in the control group to be higher in P360 than in P28. In P28, under OF and FS exposure, IL-1beta-ir in the CeA remained unaltered but increased in the MeA and in the hippocampus after acute and chronic stress. In P360 no changes were observed in the IL-1beta-ir level after acute and chronic stimulation. These data demonstrate that only the levels of IL-1beta-ir in juvenile rat brains are affected by FS and OF. Additionally, there was no significant difference between FS and OF stimulation in IL-1beta-ir.
本研究的目的是调查生命中的两个时期，即出生后第28天（P28）和出生后第360天（P360），对急性和重复旷场试验（OF）或强迫游泳试验（FS）引起的海马体（CA1、CA3、齿状回-DG）和杏仁核（中央核-CeA、内侧核-MeA）中白细胞介素-1β（IL-1β）免疫反应性（-ir）变化的影响。将大鼠分为几组：非应激组、暴露于急性应激（一次15分钟）组和慢性应激组（每天15分钟，共21天）。我们发现，对照组中P360时的IL-1β-ir高于P28时。在P28时，在旷场试验和强迫游泳试验暴露下，中央杏仁核（CeA）中的IL-1β-ir保持不变，但在急性和慢性应激后，内侧杏仁核（MeA）和海马体中的IL-1β-ir增加。在P360时，急性和慢性刺激后IL-1β-ir水平未观察到变化。这些数据表明，只有幼鼠大脑中的IL-1β-ir水平受强迫游泳试验和旷场试验的影响。此外，强迫游泳试验和旷场试验刺激在IL-1β-ir方面没有显著差异。
