Badowska-Szalewska Ewa, Ludkiewicz Beata, Spodnik Jan H, Moryś Janusz
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Gdańsk, Poland.
J Neurosci Res. 2014 Nov;92(11):1446-56. doi: 10.1002/jnr.23428. Epub 2014 Jun 17.
It is believed that the impact of stress on interleukin-1β (IL-1β) depends on the ontogenetic age. This study examines the influence of acute or chronic exposure to forced-swim (FS) stress or high-light open-field (HL-OF) stimulation on the expression of IL-1β. Double immunofluorescence staining was used to reveal the density of IL-1β/NeuN (NeuN is a neuronal nuclear marker)-immunoreactive (-ir) cells in the hippocampal subfields CA1 and CA3, dentate gyrus (DG), and paraventricular nucleus (PVN) of the hypothalamus. Adult postnatal day 90 (P90) and aged (P720) rats were used in this experiment. The data showed a significant increase in the density of IL-1β/NeuN-ir cells in the CA1, CA3, DG, and PVN in P720 nonstressed rats in relation to P90 control animals. Neither FS nor HL-OF acute stimulation caused alteration in the density of IL-1β-ir neurons in any of the investigated structures in P90 and P720 rats in comparison with control groups. However, chronic FS caused a significant increase in CA3 and DG of P720 rats, and chronic HL-OF led to a significant increase in the density of IL-1β-ir neurons in the PVN of P90 rats and in all hippocampal subfields of P720 animals. These results indicate that chronic HL-OF stimulation is a factor that induces changes in the number of IL-1β-ir neurons in the PVN of adult rats, whereas both chronic FS and HL-OF are aggravating factors for the hippocampus of aged (P720) animals.
人们认为，压力对白介素 -1β（IL -1β）的影响取决于个体发育年龄。本研究考察了急性或慢性暴露于强迫游泳（FS）应激或强光旷场（HL - OF）刺激对IL -1β表达的影响。采用双重免疫荧光染色来揭示海马CA1和CA3亚区、齿状回（DG）以及下丘脑室旁核（PVN）中IL -1β/NeuN（NeuN是一种神经元核标记物）免疫反应性（- ir）细胞的密度。本实验使用了出生后第90天（P90）的成年大鼠和老年（P720）大鼠。数据显示，与P90对照动物相比，P720未应激大鼠的CA1、CA3、DG和PVN中IL -1β/NeuN - ir细胞密度显著增加。与对照组相比，FS和HL - OF急性刺激均未导致P90和P720大鼠任何研究结构中IL -1β - ir神经元密度发生改变。然而，慢性FS导致P720大鼠的CA3和DG中IL -1β - ir神经元密度显著增加，慢性HL - OF导致P90大鼠PVN中以及P720动物所有海马亚区中IL -1β - ir神经元密度显著增加。这些结果表明，慢性HL - OF刺激是诱导成年大鼠PVN中IL -1β - ir神经元数量变化的一个因素，而慢性FS和HL - OF都是老年（P720）动物海马的加重因素。
