Savino W, Manganella G, Verley J M, Wolff A, Berrih S, Levasseur P, Binet J P, Dardenne M, Bach J F
J Clin Invest. 1985 Sep;76(3):1140-6. doi: 10.1172/JCI112069.
17 thymomas were studied by indirect immunofluorescence for the presence of thymic hormones and antigens of the major histocompatibility complex (MHC). The thymoma epithelial cells (specifically identified by their keratin content) contained thymic hormones (thymulin and thymosin alpha 1), a finding corroborated by the observation of elevated thymulin serum levels. In contrast with normal or hyperplastic thymuses, thymoma epithelial cells did not express HLA-DR and HLA-DC antigens as assessed by immunofluorescence as well as immunoblot analyses. Conversely, MHC class I antigens (HLA-ABC) were normally expressed. Thus, we conclude that thymoma epithelial cells are endocrinologically active but are defective for the expression of some MHC products (class II molecules) known to play an essential role in intrathymic T cell differentiation.
通过间接免疫荧光法对17例胸腺瘤进行研究，以检测胸腺激素和主要组织相容性复合体（MHC）抗原的存在情况。胸腺瘤上皮细胞（通过其角蛋白含量特异性鉴定）含有胸腺激素（胸腺素和胸腺肽α1），血清胸腺素水平升高的观察结果证实了这一发现。与正常或增生性胸腺相反，通过免疫荧光以及免疫印迹分析评估，胸腺瘤上皮细胞不表达HLA-DR和HLA-DC抗原。相反，MHC I类抗原（HLA-ABC）正常表达。因此，我们得出结论，胸腺瘤上皮细胞具有内分泌活性，但在表达某些已知在胸腺内T细胞分化中起重要作用的MHC产物（II类分子）方面存在缺陷。
