Ke Shao-Ying, Ma Yan-Zhi
Department of Life Sciences, Tangshan Normal College, Tangshan 063000, China.
Zhong Yao Cai. 2013 Apr;36(4):525-7.
To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..
The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC.
The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased.
DB3 and DB12 can be further studied as reproduction materials.
为筛选耐盐耐旱菘蓝品种提供参考依据。
以不同四倍体菘蓝组培苗为试验材料，以二倍体为对照，培养30天。分别用2 g/L NaCl和15% PEG-6000进行胁迫处理12小时，然后倒掉溶液，材料继续培养7天。采用高效液相色谱法测定靛玉红和靛蓝的含量。
PEG处理的DB3、DB5和DB12以及NaCl处理的DB2、DB3和DB12中靛蓝和靛玉红的含量增加。
DB3和DB12可作为繁殖材料作进一步研究。
