[Effects of PEG and NaCl stress on the contents of indigotin and indirubin in Isatis indigotica tetraploids].

OBJECTIVE

To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..

METHODS

The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC.

RESULTS

The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased.

CONCLUSION

DB3 and DB12 can be further studied as reproduction materials.