Chen Ye, Fan Chun-Lin, Wang Ying, Zhang Xiao-Qi, Huang Xiao-Jun, Ye Wen-Cai
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2091-2096. doi: 10.19540/j.cnki.cjcmm.20180116.001.
Nineteen compounds were isolated from the roots of Isatis indigotica through silica gel, Sephadex LH-20, ODS and pre-HPLC chromatography technique. Their structures were elucidated by the MS and NMR spectra as 7-hydroxydeoxyvasicinone (1), (1H-indol-3-yl) oxoacetamide (2), 1-methoxy-1H-indole-3-acetonitrile (3), arvelexin (4), 1H-indole-3-acetonitrile (5), 1H-indole-3-aldehyde (6), 1H-indole-3-acetic acid (7), 2,3-dihydro-4-hydroxy-2-oxo-indole-3-acetonitrile (8), deoxyvasicinone (9), indigotiisocoumarin A (10), cycloanthranilylproline (11), quinazoline-2,4(1H,3H)-diones (12), indirubin(13), (+)-pinoresinol (14), (+)-epipinoresinol (15), burselignan (16), (+)-isolariciresinol (17),vanillic acid (18) and 5-hydroxymaltol (19). Among them, compound 1 is a new natural product, and compound 2, 14, 15, 18 and 19 were isolated from the title plant for the first time.
通过硅胶、葡聚糖凝胶LH - 20、ODS和制备型高效液相色谱技术，从菘蓝根部分离出19种化合物。通过质谱和核磁共振光谱确定它们的结构分别为7 - 羟基脱氧瓦西酮（1）、（1H - 吲哚 - 3 - 基）氧代乙酰胺（2）、1 - 甲氧基 - 1H - 吲哚 - 3 - 乙腈（3）、阿韦勒辛（4）、1H - 吲哚 - 3 - 乙腈（5）、1H - 吲哚 - 3 - 甲醛（6）、1H - 吲哚 - 3 - 乙酸（7）、2,3 - 二氢 - 4 - 羟基 - 2 - 氧代吲哚 - 3 - 乙腈（8）、脱氧瓦西酮（9）、靛蓝异香豆素A（10）、环邻氨基甲酰基脯氨酸（11）、喹唑啉 - 2,4(1H,3H) - 二酮（12）、靛玉红（13）、(+) - 松脂醇（14）、(+) - 表松脂醇（15）、刺囊酸木脂素（16）、(+) - 异落叶松脂素（17）、香草酸（18）和5 - 羟基麦芽酚（19）。其中，化合物1是一种新的天然产物，化合物2、14、15、18和19首次从该植物中分离得到。
