一种抗合成肽单克隆抗体对纤维蛋白和纤维蛋白原的鉴别
Discrimination between fibrin and fibrinogen by a monoclonal antibody against a synthetic peptide.
作者信息
Scheefers-Borchel U, Müller-Berghaus G, Fuhge P, Eberle R, Heimburger N
出版信息
Proc Natl Acad Sci U S A. 1985 Oct;82(20):7091-5. doi: 10.1073/pnas.82.20.7091.
Abstract
Circulating soluble fibrin, observed in the blood of patients with ongoing intravascular coagulation, is generated from the plasma protein fibrinogen by the limited proteolytic action of thrombin. We report the production of a monoclonal antibody that discriminates between fibrin and fibrinogen in blood. The synthetic hexapeptide Gly-Pro-Arg-Val-Val-Glu, representing the amino terminus of the alpha chain of human fibrin, was used as immunogen. This hexapeptide is located within the A alpha chain of fibrinogen but becomes the amino terminus of the fibrin alpha chain, after fibrinopeptide A is removed by the action of thrombin, and thus becomes accessible for antibody binding. The monoclonal antibody we have prepared can discriminate between fibrin and fibrinogen and thus can be used in assay systems to quantitate soluble fibrin or, potentially, to image fibrin-rich thrombi.
摘要
在持续发生血管内凝血的患者血液中观察到的循环可溶性纤维蛋白，是由血浆蛋白纤维蛋白原在凝血酶的有限蛋白水解作用下产生的。我们报告了一种可区分血液中纤维蛋白和纤维蛋白原的单克隆抗体的产生。代表人类纤维蛋白α链氨基末端的合成六肽甘氨酸-脯氨酸-精氨酸-缬氨酸-缬氨酸-谷氨酸被用作免疫原。该六肽位于纤维蛋白原的Aα链内，但在纤维蛋白肽A被凝血酶作用去除后，成为纤维蛋白α链的氨基末端，因此可用于抗体结合。我们制备的单克隆抗体可以区分纤维蛋白和纤维蛋白原，因此可用于检测系统中定量可溶性纤维蛋白，或者可能用于对富含纤维蛋白的血栓进行成像。
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