Artificial Cell Membrane System Group, Kanagawa Academy of Science and Technology (KAST) , 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan.
Anal Chem. 2013 Nov 19;85(22):10913-9. doi: 10.1021/ac402299z. Epub 2013 Nov 5.
This paper describes the rapid and repetitive formation of planar lipid bilayers via a mechanical droplet contact method for high-throughput ion channel analysis. In this method, first, an aqueous droplet delivered in a lipid-in-oil solution is mechanically divided into two small droplets. Second, the two small droplets contact each other, resulting in the lipid bilayer formation. Third, an ion channel is immediately reconstituted into the bilayer and the transmembrane current signals are measured. By repeating this procedure, massive data sets of the channel signals can be obtained. This method allowed us to perform statistical analysis of α-hemolysin conductance (n = 256 within 30 min) and channel inhibition experiments by contacting different types of the droplets in a short time frame.
本文描述了一种通过机械液滴接触方法快速重复形成平面脂质双层的方法，用于高通量离子通道分析。在该方法中，首先，将在油相溶液中输送的水性液滴机械地分成两个小液滴。其次，两个小液滴相互接触，导致脂质双层的形成。第三，离子通道立即重新组装到双层中，并测量跨膜电流信号。通过重复此过程，可以获得大量的通道信号数据集。该方法允许我们通过在短时间内接触不同类型的液滴来进行α-溶血素电导的统计分析（n = 256，在 30 分钟内）和通道抑制实验。
