Yasuga Hiroki, Inoue Kosuke, Kawano Ryuji, Takinoue Masahiro, Osaki Toshihisa, Kamiya Koki, Miki Norihisa, Takeuchi Shoji
Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology, Kawasaki, Japan.
School of Integrated Design Engineering, Keio University, Yokohama, Japan.
PLoS One. 2017 Jul 10;12(7):e0180876. doi: 10.1371/journal.pone.0180876. eCollection 2017.
DNA logic circuits utilizing DNA hybridization and/or enzymatic reactions have drawn increasing attention for their potential applications in the diagnosis and treatment of cellular diseases. The compartmentalization of such a system into a microdroplet considerably helps to precisely regulate local interactions and reactions between molecules. In this study, we introduced a relay approach for enabling the transfer of DNA from one droplet to another to implement multi-step sequential logic operations. We proposed electrical fusion and mechanical splitting of droplets to facilitate the DNA flow at the inputs, logic operation, output, and serial connection between two logic gates. We developed Negative-OR operations integrated by a serial connection of the OR gate and NOT gate incorporated in a series of droplets. The four types of input defined by the presence/absence of DNA in the input droplet pair were correctly reflected in the readout at the Negative-OR gate. The proposed approach potentially allows for serial and parallel logic operations that could be used for complex diagnostic applications.
利用DNA杂交和/或酶促反应的DNA逻辑电路因其在细胞疾病诊断和治疗中的潜在应用而受到越来越多的关注。将这样一个系统分隔到微滴中极大地有助于精确调节分子之间的局部相互作用和反应。在本研究中，我们引入了一种中继方法，用于实现DNA从一个液滴转移到另一个液滴，以执行多步顺序逻辑操作。我们提出了液滴的电融合和机械分裂，以促进DNA在输入、逻辑操作、输出以及两个逻辑门之间的串行连接处的流动。我们开发了通过一系列液滴中并入的或门和非门的串行连接集成的或非操作。由输入液滴对中DNA的存在与否定义的四种类型的输入在或非门的读出中得到了正确反映。所提出的方法可能允许进行串行和并行逻辑操作，可用于复杂的诊断应用。
