State Key Laboratory of Chemo/Biosensing and Chemometrics, Department of Chemistry, Hunan University, Changsha 410082, People's Republic of China; Changsha Municipal Drainage Co.,Ltd., Changsha 410003, People's Republic of China.
Biosens Bioelectron. 2014 Feb 15;52:427-32. doi: 10.1016/j.bios.2013.08.026. Epub 2013 Sep 11.
A wireless, remote query octachlorostyrene (OCS) biosensor was fabricated by coating a mass-sensitive magnetoelastic ribbon with anti-OCS antibody. In response to a time-varying magnetic field, the magnetoelastic sensor mechanically vibrates at a characteristic resonance frequency which inversely depends on the sensor mass loading. As the magnetoelastic film is magnetostrictive itself, the vibrations launch magnetic flux that can be remotely detected using a pickup coil. Au nanoparticles (NPs) were used to amplify the mass loading. In a sample solution containing OCS target and OCS-modified AuNPs (OCS-AuNPs), both OCS and OCS-AuNPs react with the anti-OCS antibody immobilized on the sensor surface in a competition mode. The bound OCS-AuNPs amount is inversely proportional to the OCS target concentration. The reduction of bound OCS-AuNPs induced by free OCS results in significant change in mass loading, which amplifies the responses. The biosensor demonstrates a linear shift in resonance frequency with OCS concentration between 7.4 μM and 9 nM, with a detection limit of 2.8 nM.
一种无线、远程查询的八氯代二苯并-对-二恶英（OCS）生物传感器是通过在质量敏感的磁弹性带上涂覆抗 OCS 抗体来制备的。在时变磁场的作用下，磁弹性传感器以特征共振频率机械振动，该频率与传感器的质量负载成反比。由于磁弹性膜本身具有磁致伸缩性，因此振动会产生磁场，该磁场可以使用拾波线圈进行远程检测。金纳米粒子（NPs）被用来放大质量负载。在含有 OCS 靶标和 OCS 修饰的 AuNPs（OCS-AuNPs）的样品溶液中，OCS 和 OCS-AuNPs 都与固定在传感器表面的抗 OCS 抗体在竞争模式下反应。结合的 OCS-AuNPs 量与 OCS 靶标浓度成反比。游离 OCS 引起的结合 OCS-AuNPs 的减少导致质量负载的显著变化，从而放大了响应。该生物传感器在 7.4 μM 至 9 nM 的 OCS 浓度范围内表现出共振频率的线性偏移，检测限为 2.8 nM。
