MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education & College of Information Engineering, Taiyuan University of Technology, Jinzhong, 030600, China.
Sci Rep. 2017 Nov 15;7(1):15626. doi: 10.1038/s41598-017-15908-2.
A wireless magnetoelastic (ME) biosensor immobilized with E2 glycoprotein was first developed to detect classical swine fever virus (CSFV) E2 antibody. The detection principle is that a sandwich complex of CSFV E2 - rabbit anti-CSFV E2 antibody - alkaline phosphatase (AP) conjugated goat anti-rabbit IgG formed on the ME sensor surface, with biocatalytic precipitation used to amplify the mass change of antigen-antibody specific binding reaction, induces a significant change in resonance frequency of the biosensor. Due to its magnetostrictive feature, the resonance vibrations and resonance frequency can be actuated and wirelessly monitored through magnetic fields. The experimental results show that resonance frequency shift increases with the augmentation of the CSFV E2 antibody concentration. Scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and fluorescence microscopy analysis proved that the modification and detection process were successful. The biosensor shows a linear response to the logarithm of CSFV E2 antibody concentrations ranging from 5 ng/mL to 10 μg/mL, with a detection limit (LOD) of 2.466 ng/mL and the sensitivity of 56.2 Hz/μg·mL. The study provides a low-cost yet highly-sensitive and wireless method for selective detection of CSFV E2 antibody.
一种基于无线磁弹（ME）生物传感器的 E2 糖蛋白固定化方法，首次被开发用于检测经典猪瘟病毒（CSFV）E2 抗体。其检测原理是在 ME 传感器表面形成 CSFV E2-兔抗 CSFV E2 抗体-碱性磷酸酶（AP）缀合山羊抗兔 IgG 的三明治复合物，利用生物催化沉淀来放大抗原-抗体特异性结合反应的质量变化，从而引起生物传感器共振频率的显著变化。由于其磁致伸缩特性，共振振动和共振频率可以通过磁场进行驱动和无线监测。实验结果表明，随着 CSFV E2 抗体浓度的增加，共振频率的偏移也随之增加。扫描电子显微镜（SEM）、能谱（EDS）和荧光显微镜分析证明了修饰和检测过程的成功。该生物传感器对 CSFV E2 抗体浓度的对数呈现出线性响应，范围从 5ng/mL 到 10μg/mL，检测限（LOD）为 2.466ng/mL，灵敏度为 56.2Hz/μg·mL。该研究为选择性检测 CSFV E2 抗体提供了一种低成本、高灵敏度且无线的方法。
