Sakamoto N, Ono E, Kida H, Yanagawa R
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Jul;259(4):557-63. doi: 10.1016/s0176-6724(85)80088-2.
Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.
单克隆抗体最初是针对从问号钩端螺旋体血清型克雷马斯托斯菌株京都分离出的一种钩端螺旋体属特异性蛋白质抗原（GP-Ag）产生的。代表性单克隆抗体（GP-7）不凝集克雷马斯托斯京都菌株，但在酶联免疫吸附测定（ELISA）中与经Triton X-100处理的克雷马斯托斯京都菌株、安达马纳菌株、帕托克菌株和浦和菌株发生反应，这表明该单克隆抗体识别GP-Ag的属特异性决定簇。使用碘化抗体和过氧化物酶标记抗体通过免疫电子显微镜研究了GP-Ag的定位。GP-7识别的GP-Ag的属特异性决定簇位于钩端螺旋体外膜的亚表面。
