Masuzawa T, Kumagai M, Shimizu T, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Clin Microbiol. 1988 Nov;26(11):2332-7. doi: 10.1128/jcm.26.11.2332-2337.1988.
Leptospira interrogans serovar lai was identified in China in 1966 as a new serovar of the icterohaemorrhagiae serogroup by cross-absorption tests. In this study, we established three hybridoma cell lines producing monoclonal antibodies (MAbs) of the immunoglobulin G3 subclass (LW1, LW2, and LW3) and of the immunoglobulin M class (LW4a) against serovar lai strain 017 by the cell fusion technique. Immunological reactivities of the MAbs were determined by the microscopic agglutination test, enzyme-linked immunosorbent assay (ELISA), and indirect immunofluorescence assay. MAbs LW1 and LW3 agglutinated cells of serovars lai, birkini, and gem of serogroup icterohaemorrhagiae. LW2 agglutinated various serovars of serogroup icterohaemorrhagiae, except for serovar tonkini. LW4a reacted positively with various Leptospira species, including a new species, Leptospira parva, in the ELISA and indirect immunofluorescence assay. However, LW4a did not react with Leptonema illini 3055. The results of an inhibition ELISA with heated outer envelope (OE) or periodate-oxidized OE suggested that these MAbs recognize a carbohydrate moiety of the OE as the antigenic determinant.
1966年，问号钩端螺旋体赖型在中国通过交叉吸收试验被鉴定为出血性黄疸血清群的一个新血清型。在本研究中，我们通过细胞融合技术建立了三个杂交瘤细胞系，分别产生针对赖型菌株017的免疫球蛋白G3亚类（LW1、LW2和LW3）和免疫球蛋白M类（LW4a）的单克隆抗体。通过显微镜凝集试验、酶联免疫吸附测定（ELISA）和间接免疫荧光测定来确定单克隆抗体的免疫反应性。单克隆抗体LW1和LW3凝集出血性黄疸血清群赖型、伯氏型和杰氏型的细胞。LW2凝集出血性黄疸血清群的各种血清型，但不包括汤氏型。在ELISA和间接免疫荧光测定中，LW4a与包括新种小钩端螺旋体在内的各种钩端螺旋体物种呈阳性反应。然而，LW4a与伊利诺伊细螺旋体3055不发生反应。用加热的外膜（OE）或高碘酸盐氧化的OE进行抑制ELISA的结果表明，这些单克隆抗体识别OE的碳水化合物部分作为抗原决定簇。
