Rubakova E I, Ermakov G P, Fontalin L N, Nesterenko V G, Belaia Iu A
Zh Mikrobiol Epidemiol Immunobiol. 1985 Jul(7):77-80.
The method for the determination of the number of cells synthetizing antibodies to S. flexneri VI O-antigen in the spleen of mice has been developed. Primary immune response to this antigen has been studied with the use of the new method. Immune response to the optimum immunogenic dose of O-antigen has a manifest variable character. The intensity of primary immune response has been shown to rise with the increase of the dose of O-antigen from 0.004 to 50 micrograms. The preliminary injection of 200 micrograms of O-antigen, followed by the injection of cyclophosphamide 2 days later, leads to the development of specific immunological tolerance to O-antigen in experimental animals.
已开发出测定小鼠脾脏中合成针对福氏志贺菌VI型O抗原抗体的细胞数量的方法。利用该新方法研究了对该抗原的初次免疫反应。对O抗原最佳免疫原剂量的免疫反应具有明显的变异性。已表明，随着O抗原剂量从0.004微克增加到50微克，初次免疫反应的强度会升高。预先注射200微克O抗原，然后在2天后注射环磷酰胺，会导致实验动物对O抗原产生特异性免疫耐受。
